background and objectives: significant progress has been made in treatment of hemophilia. ex-vivo gene therapy is going popular due to the capability of this method in using isogenic cells for genetic manipulation and reintroducing them into same host after proliferation. most gene therapy techniques use viral vectors, which usually harbor a strong and non-specific promoter (e.g. cmv early prom...

the role of microbiota in health and disease is the subject of rigorous investigation. several studies have demonstrated that microbiota and the pattern-recognition receptors contribute to intestinal tumourigenesis; the exact mechanism of which is still obscure. myd88 is the downstream effector of all toll-like receptors (tlrs) except tlr3. however, the alternative myd88-independent pathway is ...

background & aims of the study: heavy metals contaminate drinking water, air and soils. it is very harmful for humans and other live organisms. phytoremediation is one of the direct bio-remediation methods for heavy metal removal from polluted water and soils. this method is accepted environmentally, economically and aesthetically. the purpose of this study was to assess tamarix hispida capacit...

production of PtdIns-3,4,5-P 3. In vitro, this lipid can be produced by the p170/mCpk-type PI3K that phosphory-Montreal Neurological Institute McGill University lates PtdIns-4-P at the D-3 position (Figure 1) or by a kinase that phosphorylates PtdIns-3-P at the D-4 posi-Montreal, Quebec H3A 2B4 Canada tion (not shown). Thus, the relative levels of PtdIns-3,4-P2 and PtdIns-3,4,5-P3 are independe...

objective(s):n-myc downstream regulated gene 2 (ndrg2) is a candidate gene for tumor suppression. the expression of ndrg2 is down-regulated in several tumors including lung cancer. the aim of this study was to explore the effect of ndrg2 overexpression on invasion, migration, and enzymatic activity of matrix metalloproteinase-2 (mmp-2) and -9 (mmp-9) in human lung adenocarcinoma a549 cells.  ma...

The evolutionarily conserved checkpoint protein kinase, TOR (target of rapamycin), has emerged as a major effector of cell growth and proliferation via the regulation of protein synthesis. Work in the last decade clearly demonstrates that TOR controls protein synthesis through a stunning number of downstream targets. Some of the targets are phosphorylated directly by TOR, but many are phosphory...

The family of Toll-like receptors (TLRs) senses conserved structures found in a broad range of pathogens, causing innate immune responses that include the production of inflammatory cytokines, chemokines and interferons. The signal transduction is initiated from the Toll/interleukin-1 receptor (TIR) domain of TLRs after pathogen recognition. Almost all TLRs use a TIR-containing adapter MyD88 to...