نتایج جستجو برای: down pcr
تعداد نتایج: 377022 فیلتر نتایج به سال:
Staphylococcal enterotoxin B (SEB), an exotoxin produced by staphylococcal aureus, is commonly associated with food poisoning and capable of triggering flu-like symptoms resembling those of endotoxins – such as lipopolysaccharide (LPS) released from gram-negative bacterial cell walls. By comparing the host response to these distinct toxins on a genetic level, a set of genes can be identified to...
ubiquitin - proteasome system (ups), the major protein degradation pathway in the cells, typically degrades short - lived and damaged proteins and regulates growth and stress responses. this pathway is altered in various cancers, including acute lymphoblastic leukemia (all). all begins with a change in bone marrow cells and is the most common type of leukemia in children under 15 years. ube2q1 ...
چکیده زمینه و هدف: اکثر ژنهای خانهدار، سرکوبگر تومور، و تقریبا 40% ژنهای اختصاصی بافتها حاوی توالی غنی از G+C در نواحی پروموتری خود میباشند که تکثیر آن با مشکلات زیادی همراه است. ژنAstrocyte elevated gene -1(AEG-1) دارای نقش عملکردی در چندین مرحله از پیشرفت تومور از جمله، ترانسفورماسیون، آپوپتوزیس، متاستاز، مقاومت به شیمی درمانی، رگزایی و پیری سلول میباشد. مواد و روش کار: این مطالعه، به ب...
in this study, several designed creb1-specific shrnas cloned into lentiviral transfer vectors were screened in293t by transient transfection assays. relative creb1 expression was determined by relative qrt-pcr. all of the designed shrnas gave some level of knockdown. one shrna was more effective for knocking down the specific gene among others. the predicted sirnas activities as determined by r...
چکیده- خستگی عبارت است از پدیده ی شکست بر اثر تکرار بار، که تنش ایجاد شده توسط این بار ممکن است حتی به مراتب کوچک تر از مقاومت نهایی مصالح باشد .ترکهای ناشی از خستگی، در مقاطع آسفالتی، با توجه به مکان پیدایش و نحوه ی گسترش آنها دو دسته است .دستهی اول ترک هایی است که در بخش پایینی مقطع آسفالتی پدید می آیند و سپس با تکرار بار بهسمت بالا گسترش مییابند. پیدایش این نوع ترک ها در مقاطع آسفالتی با ضخا...
We report three cases of asymptomatic celiac disease identified in children with Down syndrome after being screened at around twenty-four months of age. These cases raise the question as to what age is screening for celiac disease indicated in a child with Down syndrome and no symptoms.
A differential-display PCR procedure identified the capsular assembly gene kpsD after Escherichia coli type 1 fimbrial binding to mannose-coated Sepharose beads. Limiting-dilution reverse-transcribed PCRs confirmed down-regulation of the kpsD gene, and Northern blot and lacZ fusion analyses showed down-regulation of the kpsFEDUCS region 1 operon. KpsD protein levels fell, and an agglutination t...
Oral-wash samples obtained during 113 episodes of suspected Pneumocystis pneumonia (PCP) in human immunodeficiency virus-infected patients were tested by use of a quantitative touch-down PCR (QTD PCR) assay. QTD PCR had a sensitivity of 88% and a specificity of 85%. Treatment for PCP prior to oral wash collection had an impact on the sensitivity, and PCR-positive oral-wash samples obtained with...
We describe a convenient, cost-effective and flexible medium-throughput single nucleotide polymorphism (SNP) genotyping method, Multiplex SNP-SCALE, which enables the simultaneous amplification by polymerase chain reaction (PCR) of up to 25 (or potentially more) loci followed by electrophoresis in an automated DNA sequencer. We extended the original SNP-SCALE method to include (i) use of a comm...
PURPOSE The aim of this study was to examine the significance of EBP50 (ezrin-radixin-moesin binding phosphoprotein 50) expression in esophageal squamous cell carcinoma (ESCC). MATERIALS AND METHODS Real-time PCR (qRT-PCR), western blotting, and immunohistochemical staining were performed to detect EBP50 expression in pairs of ESCCs and matched non-tumor tissues, and the relationships between...
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