We have developed an in vitro system in which repair of DNA double-strand breaks is performed by purified proteins of Escherichia coli. A segment was deleted from a circular duplex DNA molecule by restriction at two sites. 3' single-stranded overhangs were introduced at both ends of the remaining linear fragment. In a first step, RecA protein catalyzed the formation of a D-loop between one sing...

control of three phase inverters has been an important issue in recent years. this paper proposes active disturbance rejection control (adrc) scheme for controlling three phase inverters. the main advantage of adrc that motivated us for using this controller for inverter is that it is model independent.  this controller is used in a double loop structure including an inner current loop and oute...

Leishmania mexicana can cause both localized (LCL) and diffuse (DCL) cutaneous leishmaniasis, yet little is known about factors regulating disease severity in these patients. We analyzed if the disease was associated with single nucleotide polymorphisms (SNPs) in IL-1β (-511), CXCL8 (-251) and/or the inhibitor IL-1RA (+2018) in 58 Mexican mestizo patients with LCL, 6 with DCL and 123 control ca...

[1] Sulfate, nitrate, and chloride are major water-soluble anions in soils of the McMurdo Dry Valleys (MDV), Antarctica. We measured the chloride stable isotope composition of 40 soil samples from 16 different soil profiles. The soil profiles come from three different microclimate zones in the MDV: a coastal thaw zone, which fosters a seasonally saturated active layer; a stable upland zone, in ...

Intermediates in the replication of circular and linear M13 double-stranded DNA by bacteriophage T7 proteins have been examined by electron microscopy. Synthesis generated double-stranded DNA molecules containing a single replication fork with a linear duplex tail. A complex presumably consisting of T7 DNA polymerase and gene 4 helicase/primase molecules was present at the fork together with a ...

Hwang and Xu defined equivalent double-loop networks and gave one such result showing that the L-shapes of the two equivalent networks are recombinations of three rectangles. Recently, Rödseth gave an elegant algebraic theorem for equivalent multi-loop networks. We show that its double-loop version yields equivalent networks of the 3rectangle version. We also show that other seemingly different...

Drawing on Henri Meschonnic’s notion of an “inscient ethics,” and putting “inscience” into dialogue with the old ideal of a “science” of translation, the article explores the collective socio-affective ecologies that organize and regulate social and professional norms and values of translation below the level of conscious awareness—as the true underlying structure not only of “subjectivity” (so...

A novel template design for single-molecule sequencing is introduced, a structure we refer to as a SMRTbell template. This structure consists of a double-stranded portion, containing the insert of interest, and a single-stranded hairpin loop on either end, which provides a site for primer binding. Structurally, this format resembles a linear double-stranded molecule, and yet it is topologically...

Many applications with stream processing behavior contain one or more loops with an unknown number of iterations. These loops have to be parallelized in order to utilize the maximum capacity of an embedded multiprocessor platform and thus increase the total throughput. This thesis presents a method to automatically extract a parallel task graph based on function level parallelism from a sequent...

The statistical mechanics of DNA denaturation under fixed linking number is qualitatively different from that of unconstrained DNA. Quantitatively different melting scenarios are reached from two alternative assumptions, namely, that the denatured loops are formed at the expense of (i) overtwist or (ii) supercoils. Recent work has shown that the supercoiling mechanism results in a picture simil...