the plasmid palca1sifn containing cdna that encodes the human interferon a-2b was obtained from the atcc(no. 531667). in this system the expression of the gene is under the control of an alca promoter. alca p is a specific promoter for expression of different genes in aspergillusfilamentous. in this plasmid the coding region of ifn?-2b is preceded by the coding region of a synthetic signal pept...

BACKGROUND Human growth hormone (hGH) is a single-chain polypeptide that participates in a wide range of biological functions such as metabolism of proteins, carbohydrates and lipids as well as in growth, development and immunity. Growth hormone deficiency in human occurs both in children and adults. The routine treatment for this condition is administration of recombinant human growth hormone ...

Using genetic immunisation of mice, we produced antibodies against chicken interleukin-2 (ChIL-2), the first produced against a non-mammalian interleukin. After a final injection with a recombinant ChIL-2 protein, two stable hybridoma cell lines were established which secreted monoclonal antibodies (MAbs) against this cytokine. Specific binding of the two MAbs to recombinant ChIL-2 produced by ...

Antibodies to Gardnerella vaginalis were raised in rabbits. Nine antisera that reacted with their immunising strains, but not with the remaining eight strains, were used to develop a serotyping scheme. A dot blotting technique was used, and complexes of antigen and antibody were visualised using anti-rabbit immunoglobulin linked to alkaline phosphatase. Of 91 clinical isolates used to evaluate ...

Randomly selected goat sera from north-western, central, and south-western regions of Uganda were analyzed parasitologically and serologically for evidence of anaplasmosis. Prevalence rates of 3.2% by parasitemia, 4.8% by card-agglutination test, and 12.9% by DOT-ELISA combined with western blotting were established. Parasitologically positive samples were consistently serologically positive. P...

長年，アレルゲンの解析手法としてSDS-PAGEとWestern blottingが用いられてきた．この場合，タンパク質をできるだけ可溶化するために，SDSなどの変性剤を高濃度で添加することが多い．筆者らはこの手法を用いて，甲殻類アレルゲンの種特異的反応性の違いを検出した．ところが，別の患者血清では，この方法ではバンドが全く検出されなかった．そこでこの患者血清をdot blottingで調べたところ，生では強く反応したが，80°Cで15分加熱すると反応が失われた．そこで，生の試料をdot blotしたPVDF膜を0.1％SDSを含む電気泳動バッファー中で1時間インキュベートしたところ反応性は弱まったが，完全には反応が消失していなかった．そこで，0.1％SDSを含むSDS抽出液を用いてSDS-PAGEを行ったところ，トロポミオシンのバンドが弱く反応した．甲殻類アレルゲンのSDS変性を低...

The synthesis and binding integrity of an ErbB2-binding modified DNA aptamer (E2Ap) with 5-naphtyl-20deoxyuridine is confirmed by electrospray ionization mass spectrometry and by a P dot blotting experiment. E2Ap degradation in a conventional Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC) condition is efficiently prevented by using tris(3-hydroxypropyltriazolylmethyl)amine (THPTA). The immo...

The aberrant expression of long noncoding RNAs (lncRNAs) has great impacts on cancer origination and progression. In the current study, a newly found lncRNA Z38, which was identified through combining experiments of suppression subtractive hybridization (SSH) and reverse dot-blotting, was found to have high expression in breast cancer. More importantly, inhibiting Z38 expression by gene silenci...