Bone marrow (BM) chimeras prepared by complete recipient ablation (A-->B) exhibit donor-specific tolerance, yet survival is often limited by graft-versus-host disease (GVHD). Negative selection of potentially donor-reactive T cells, as assessed by relative T-cell receptor (TCR)-Vbeta expression, is dependent on donor BM-derived deleting ligands. Mixed chimerism and tolerance for both donor and ...

The application of allogeneic stem cell transplantation (alloSCT) is limited by graft-versus-host disease (GVHD). GVHD can be divided into acute and chronic forms that likely have different requirements for initiation and pathogenesis mechanisms. In prior studies we demonstrated that residual host antigen-presenting cells (APCs) were required to initiate acute GVHD (aGVHD) mediated by CD8 T cel...

The acanthocephalan parasite Pomphorhynchus laevis (Müller, 1776) uses freshwater amphipods as its intermediate host. In order to complete the life cycle, the infected amphipod must be consumed by a fish, where the acanthocephalan will mature and reproduce. Parasite transmission, and therefore fitness, could be enhanced if infected amphipods fail to detect or avoid predatory fish. We compared t...

1. A new assay of graft-host interactions has been presented. It consists of the early finding of liver infiltrates in responsive and unresponsive homologous recipients 1 to 3 days after the intravenous injection of competent lymphoid tissue in the mouse. 2. Evidence is presented that infiltrates are of donor origin, and are influenced by a homologous antigenic environment. They can be quantita...

purpose: to evaluate exposure rate following wrapping hydroxyapatite implant in mersilene mesh and donor sclera. methods: in a prospective comparative interventional case-series 60 consecutive patients underwent primary hydroxyapatite orbital implant wrapped in mersilene mesh or donor sclera. results: thirty patients (21 male and 10 female) underwent implantation with mersilene mesh and 30 pati...

Skeletal myoblast transplantation is a potential treatment for congestive heart failure. To study the functional activity of both donor and host myocytes following transplantation, skeletal myoblasts expressing an enhanced green fluorescent protein (EGFP) transgene were transplanted into hearts of nontransgenic recipients, and changes in intracellular calcium concentration ([Ca2+]i) were monito...

Graft versus host disease (GVHD) is a major complication of allogeneic hematopoietic stem cell transplantation, leading to signi®cant morbidity and mortality. Host-derived TNF-a play a role in the induction of allo-reactive donor T cell activation and the pathogenesis of GVHD. On the other hand, the precise role of donor-derived TNF-a in GVHD remains unclear. To elucidate this issue, we designe...

Clinical trials and experimental studies have demonstrated that donor T cells can play a critical role in preventing allogeneic marrow graft rejection. Results of a previous study showed that donor T cells were most effective for preventing rejection when they recognize an alloantigen expressed by recipient T cells and can cause graft-versus-host disease (GVHD). The present study examined model...