To facilitate DNA packaging and photolytic release, o-nitrobenzyl and methacrylate functionalized PEI (P10A) was synthesized for condensing DNA into nanoparticles as small as 160 nm after radical polymerization with initiator. The gene expression following delivery of uncross-linked P10A/DNA was unchanged by photoirradiation of cells. However, exposure to photo-irradiation caused a 3-fold incre...

Jinghua Tang,1 Norman Olson,1 Paul J. Jardine,2 Shelley Grimes,2 Dwight L. Anderson,2,3,* and Timothy S. Baker1,4,* 1Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA 2Department of Diagnostic and Biological Sciences and Institute for Molecular Virology 3Department of Microbiology University of Minnesota, Minneapolis, MN 55455, USA 4Division ...

Nucleic-acid hybridization is widely used for the specific capture of complementary sequences from complex samples. It is useful for both analytical methodologies, such as array hybridization (e.g. transcriptome analysis, genetic-variation analysis), and preparative strategies such as exome sequencing and sequence-specific proteome capture and analysis (PICh, HyCCAPP). It has not generally been...

Adapting controlled release technologies to the delivery of DNA has the potential to overcome extracellular barriers that limit gene therapy. Controlled release systems can enhance gene delivery and increase the extent and duration of transgene expression relative to more traditional delivery methods (e.g., injection). These systems typically deliver vectors locally, which can avoid distributio...