نتایج جستجو برای: dna hybridization
تعداد نتایج: 543321 فیلتر نتایج به سال:
By application of modern recombinant DNA technology, especially the polymerase chain reaction (PCR)/dot-blot hybridization techniques, we have investigated the molecular basis of β-thalassemia from four different regions of Iran: central, south-east, south and north. In this study, the DNA samples were isolated from patients and for the identification of the mutations, the 6 oligonucleotide...
The combination of DNA and π-conjugated polyelectrolytes (CPEs) represents a promising approach to develop DNA hybridization biosensors, with potential applications for instance in the detection of DNA lesions and single-nucleotide polymorphisms. Here we exploit the remarkable optical properties of a cationic poly[3-(6'-(trimethylphosphonium)hexyl)thiophene-2,5-diyl] (CPT) to decipher the self-...
We consider the problem of sequence reconstruction in sequencing-by-hybridization in the presence of spectrum errors. As suggested by intuition, and reported in the literature, false-negatives (i.e., missing spectrum probes) are by far the leading cause of reconstruction failures. In a recent paper we have described an algorithm, called "threshold-theta", designed to recover from false negative...
The present research aimed to investigate through a polyphasic approach, the differences in morphological and genotypic features of two cyanobacterial strains isolated from paddy fields of northern parts of Iran, belonging to the family Nostocaceae (subsect. IV. I). Based on the reliable identification keys, the two strains were identified as Nostoc ellipsosporum (Desm.) Rabenh. ex Born. et Fla...
Hybridization of surface-bound DNA with complementary strands is the basis many biotechnological applications. Herein, structure interfacial coatings between substrate and bound a crucial element for hybridization behavior. three reactive surfaces constructing DNA-sensing platforms, namely, plain gold films on silicon, poly(bisphenolA-co-epichlorohydrin) (PBAG) brush-like bilayer structure, dib...
Nucleic acid hybridization among root-knot nematode mitochondrial DNAs can be used to identify several Meloidogyne species. Research was initiated to optimize mitochondrial DNA-based molecular diagnostics for the demanding environments likely to be encountered in field isolates. DNA hybridization using reconstituted DNA-soil mixtures revealed a loss of assay sensitivity ranging from 34% to 92% ...
Studying DNA hybridization equilibrium at atomistic length scales, either via molecular dynamics (MD) or through commonly used advanced sampling approaches, is notoriously difficult. In this work, we describe an order-parameter-based technique to calculate the free energy of hybridization, and estimate melting temperature oligomers resolution. The landscapes are reported as a function native-to...
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