tau.amegroups.com © Translational Andrology and Urology. All rights reserved. It is an honour to write the foreword to this focused issue of TAU on sperm DNA fragmentation (SDF). From my time as a first generation embryologist in the early days at the University of Cambridge with Professor Robert Edwards and in my current role at the London Women’s Clinic, I have become acutely aware of the num...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. I agree with Agarwal et al. (1) that sperm DNA fragmentation (SDF) should be included in the evaluation of male factor infertility, along with semen analysis. Although there exist divergent views on this topic, our group has routinely employed SDF (TUNEL assay) and other methods for identification of sperm DNA damage ...

A method has been developed to prepare random DNA fragments using PCR. First, two cycles are carried out at 16 degrees C with the Klenow's fragment and oligonucleotides (random primers) with random 3'-sequences and the 5'-constant part containing the site for cloning with the site-specific endonuclease. The random primers can link to any DNA site, and random DNA fragments are formed during DNA ...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. We greatly value the commentary by Dr. Gosalvez on the “Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios” by Agarwal et al. (1). The author initiated his well-written note with an overview of the structural and physiologic significance of sperm DNA on male repro...

OBJECTIVE To investigate the presence of programmed cell death in unfertilized oocytes after intracytoplasmic sperm injection (ICSI), assuming that previous apoptotic events could be correlated with the fertilization failure. DESIGN Comparison of the rate of DNA fragmentation in human oocytes at different stages of maturation soon after pick-up (control) and in unfertilized oocytes after ICSI...

DNA fragmentation into internucleosomal fragments is the best recognized biochemical event of apoptosis. Two major caspase pathways have been identified in the signal transduction leading to DNA fragmentation: the receptor pathway and the mitochondrial pathway. DNA fragmentation factor (DFF) has been identified as a major apoptotic endonuclease in the internucleosomal DNA fragmentation process....

The editors of this book, Drs. Ashok Agarwal, Ahmad Majzoub, Chak-Lam Cho and Sandro Esteves, are to be congratulated for bringing together an extensive tome on the evaluation and management of sperm DNA fragmentation. The perspectives from North America, South America, Europe, Asia and the rest of the world provide an interesting snapshot into management of the unique phenomenon of sperm DNA d...

Cleavage of DNA into internucleosomal fragments is one of the characteristics of apoptosis. However, searches for in vivo evidence of nucleosomal DNA fragmentation in leukemia cells freshly obtained from patients during chemotherapy frequently failed to reveal nucleosomal multimers (DNA ladders). It is not clear whether this type of DNA cleavage is an essential event in drug-induced apoptosis a...

Cleavage of DNA into internucleosomal fragments is one of the characteristics of apoptosis. However, searches for in vivo evidence of nucleosomah DNA fragmentation in leukemia cells freshly obtained from patients during chemotherapy frequently failed to reveal nucleosomal multimers (DNA ladders). It is not clear whether this type of DNA cleavage is an essential event in drug-induced apoptosis a...