Bacterial-based systems as live vectors for the delivery of heterologous antigens offer a number of advantages as vaccination strategies. Developments in genetic engineering have given Gram-positive lacticacid bacteria (LAB) the advantage of being used as a host expression system for antigen delivery to inducethe immune response. A fragment containing the full length of the “eprA1” ...

In this study, we were synthesized Fe3O4@LDH@Lamivudine and characterized by FT-IR spectroscopy,XRD and TEM. The interaction of this nanoparticle with CT-DNA was investigated by viscosity, circulardichroism (CD), Uv-Visible and fluorescence spectroscopy. Among all nanocarriers which applied as drugdelivery vectors, layered double hydroxides (LDHs) with exchangeable anions in t...

DNA origami structures can be programmed into arbitrary shapes with nanometer scale precision, which opens up numerous attractive opportunities to engineer novel functional materials. One intriguing possibility is to use DNA origamis for fully tunable, targeted, and triggered drug delivery. In this work, we demonstrate the coating of DNA origami nanostructures with virus capsid proteins for enh...

BACKGROUND We have previously demonstrated protective efficacy against B. melitensis using formulations of naked DNA vaccines encoding genes ialB and omp25. The present study was undertaken to further understand the immune response generated by the protective vaccination regimens and to evaluate cationic liposome adsorption as a delivery method to improve vaccine utility. METHODS The protecti...

Particle-mediated epidermal delivery (PMED) of DNA vaccines is based on the acceleration of DNA-coated gold directly into the cytoplasm and nuclei of living cells of the epidermis, facilitating DNA delivery and gene expression. Professional antigen-presenting cells and keratinocytes in the skin are both targeted, resulting in antigen presentation via direct transfection and cross-priming mechan...

PURPOSE Non-viral vectors have been widely proposed as safer alternatives to viral vectors, and cationic polymers have gained increasing attention because they can form self-assembly with DNA. Chitosan is also considered to be a good candidate for gene delivery systems, since it is already known as a biocompatible, biodegradable, and low toxic material with high cationic potential. However, low...

glioma, as a primary tumor of central nervous system, is the main cause of death in patients with brain cancer. therefore, development of an efficient strategy for treatment of glioma is worthy. the aim of the current study was to develop a srl peptide-coated dendrimer as a novel dual gene delivery system for targeting the lrp receptor, an up-regulated gene in both bbb and glioma cells. to perf...

Embryo DNA fingerprinting represents an important tool for tracking embryo-specific outcomes after multiple embryo transfer during IVF. The situation in which two embryos are transferred and only one implants represents a unique opportunity for the most well-controlled validation of markers capable of identifying competent and incompetent embryos. Specifically, this design eliminates all patien...