Background: Immunotoxins are comprised of both the cell targeting and the cell killing moieties. We previously established a new immunotoxin, i.e. Shiga toxin granulocyte macrophage-colony stimulating factor (StxA1-GM-CSF), comprises of catalytic domain of Stx, as a killing moiety and GM-CSF, as a cell targeting moiety. In this study, the ability of the immunotoxin to induce apoptosis and dou...

background: quantification of radiation-induced apoptosis in peripheral blood lymphocytes (pbls) has been proposed as a possible screening test for cancer-prone individuals and also for the prediction of normal tissue responses after radiotherapy. materials and methods: the neutral version of the comet assay (single-cell gel electrophoresis) was used 24, 48, 72 hours after irradiation with 8 gy...

background and aims. there is no report on the apoptotic impact of allium sativum l. (garlic) on the oral squamous cell carcinoma (kb); hence, this study was designed to survey the apoptotic effects of garlic fresh juice (gfj) on the kb cells. materials and methods. mtt assay (microculture tetrazolium assay) was carried out to evaluate the cytotoxicity of gfj on kb cells. furthermore, tunel (te...

Topoisomerase is one of the most important targets anti-cancer drugs. In order to develop effective and low-toxic topoisomerase inhibitors, a series xanthone derivatives have been designed synthesized using principles skeleton transition. vitro cytotoxicity assays human breast cancer (MCF-7), gastric (MGC-803), cervical cancer(Hela) cell lines, compounds showed antitumor growth activity, also l...

Two novel complexes [CuL2(H2O)2]n (1), [(ZnL2)H2O]n (2) (HL ?= ?5-(Pyrazol-1-yl)nicotinic acid) were synthesized and their vitro biological activity was systematically studied. The ability of the to bind FS-DNA measured by fluorescence spectroscopy, Stern-Volmer quenching values complex 1–2 are 0.03904 0.09522. cleavage behavior agarose gel electrophoresis showed cut pBR322 plasmid DNA. Both fl...

The adoption of new treatment modalities remains crucial as lung cancer has one the lowest survival rates, along with liver and pancreatic cancer. Bortezomib is a proteasome inhibitor that higher anticancer effect in combination therapies. Therefore, aim this study to investigate whether bortezomib could have additional when combined cyclin-dependent kinase (CDK) inhibitor-roscovitine vitro. Ap...

Conducting cell apoptosis pathways is a novel strategy in cancer treatment. This study aimed to explain that C. botrys essential oil could induce apoptosis and arrest the cell cycle in HeLa cells. Cytotoxic and apoptogenic effects of the essential oil of Jerusalem-oak (Chenopodium botrys L.), which was obtained from the aerial parts of the plant, were evaluated in HeLa cells. Cell viability was...

Epigenetic mechanisms assist in maintaining gene expression patterns and cellular properties in developing and adult tissues. The molecular pathology of disease states frequently includes perturbation of DNA and histone methylation patterns, which can activate apoptotic pathways associated with maintenance of genome integrity. This perspective focuses on the pathways linking DNA methyltransfera...

OBJECTIVE To explore the effects of Sterigmatocystin (ST), Deoxynivalenol (DON) and Aflatoxin G1 (AFG1) on apoptosis of human peripheral blood lymphocytes (HPBLs) in vitro and thus to further elucidate the putative roles of these three mycotoxins on human immunosystem. METHODS The effects of ST, DON and AFG1 on apoptosis of HPBLs were studied with cell culture, flow cytometric (FCM) DNA analy...