The present study evaluated the effi cacy of various culture media for performing the isolation and growth of the rubella virus inoculated into SIRC cells. Rubella virus RA-27/3 strain and RVi/SãoPaulo/BRA99 wild type strain (GenBank number DQ458965) were inoculated into SIRC cell line and cultivated in 199, DMEM, MEM and RPMI media. The inoculated cells when examined on phase contrast microsco...

BACKGROUND To examine the effect of the natural antimicrobial peptide human β-defensin-3 (hBD-3), on the migration of a head and neck cancer cell line in vitro using microfabrication and soft-lithographic techniques. METHODS TR146 cancer cells were seeded in Petri dishes with microfabricated wells for cell migration assays. Total 54 cell islands were used of various shape and size and experim...

Human Acellular Dermal Matrices (HADM) are employed in various reconstructive surgery procedures as scaffolds for autologous tissue regeneration. The aim of this project was to develop a new type of HADM for clinical use, composed of glycerolized reticular dermis decellularized through incubation and tilting in Dulbecco's Modified Eagle's Medium (DMEM). This manufacturing method was compared wi...

Allogenic osteochondral tissue (OCT) is used for the treatment of large cartilage defects. Typically, OCTs collected during the disease-screening period are preserved at 4°C; however, the gradual reduction in cell viability during cold preservation adversely affects transplantation outcomes. Therefore, improved storage methods that maintain the cell viability of OCTs are needed to increase the ...

Vitrification of endothelial cells (MHECT-5) has not previously been compared with controlled slow freezing methods under standardized conditions. To identify the best cryopreservation technique, we evaluated vitrification and standardized controlled-rate -1°C/minute cell freezing in a -80°C freezer and tested four cryoprotective agents (CPA), namely dimethyl sulfoxide (DMSO), ethylene glycol (...

Stimulation of protein kinase C (PKC) in a variety of cells causes enhanced catabolism of phosphatidylcholine [ 1,2]. Diacylglycerol (DAG) released from phosphatidylcholine turnover functions as a second messenger in plasma membranes activating PKC [3]. In the CNS at least, choline released extracellularly as a result of enhanced phosphatidylcholine (PC) turnover may provide free choline for ac...

Well-characterised human osteoblastic bone marrow cell cultures are a useful in vitro tool to analyse bone tissue/biomaterials interactions. In this work, human bone marrow was cultured in experimental conditions described to favour osteoblastic di!erentiation and, serially passaged cells were cultured in two widely used culture media, minimum essential medium Eagle, alpha modi"cation (a-MEM) a...

Several studies have suggested that angiotensin converting enzyme inhibitors retard the process of cataractogenesis by scavenging free oxygen radicals. The present study sought to assess the efficacy of the ACE inhibitor Lisinopril in preventing selenite-induced cataractogenesis in an experimental setting. The in vitro phase of the study was performed on lenses from wistar rats incubated for 24...

The syntheses, crystal structures, and magnetochemical characterization of four new iron clusters [Fe7O4(O2CPh)11(dmem)2] (1), [Fe7O4(O2CMe)11(dmem)2] (2), [Fe6O2(OH)4(O2CBut)8(dmem)2] (3), and [Fe3O(O2CBut)2(N3)3(dmem)2] (4) (dmemH=Me2NCH2CH2N(Me)CH2CH2OH)=2-{[2-(dimethylamino)ethyl]methylamino}ethanol) are reported. The reaction of dmemH with [Fe3O(O2CR)6(H2O)3](NO3) (R=Ph (1), Me (2), and Bu...

BACKGROUND Adipose-derived stromal (stem) cells (ASC) have been shown to be of great therapeutic use in pre-clinical studies in diverse fields, but a standard expansion method has not been established. We investigated the effects of an endothelial growth medium (EGM-2) on ASC, focusing on proliferation and differentiation potentials. METHODS ASC were cultured in EGM-2 and DMEM. Doubling time ...