The redox state of cellular thiols is widely studied because it was recently linked to many different diseases and pathologies. In this work we quantified the concentrations of protein disulfides (PSSP) and thiol-protein mixed disulfides (XSSP) in rat tissues (liver, kidney and heart) and cells (Raw 264.7) by an improved method of XSSP and PSSP determination after oxidative stress induced by di...

Introduction A s o a r t o f a q t r r d w n f t h e r e l a t i o n b e t w e e n s t r u c t u r e a n J p r o p e r t i e s o f t h e d i s u l f i d e s f o r m e d b y o x i d a t i o n o f t h i o l s u s i n g t h r o l d i s , r l f i o e i n t e r c h a n g e r e a c t i o n s , l 3 w e h a v e b e e n i n t e r e s t e d i n t h e d i s t r i b u t i o n o f p r o d u c t s o b t a i ...

We previously demonstrated that a heterotypic complex of the two rat asialoglycoprotein receptor subunits was assembled during cell-free translation (Sawyer, J. T., and D. Doyle. 1990. Proc. Natl. Acad. Sci. USA. 87:4854-4858). We have characterized this system further by analyzing polypeptide interactions under both reducing and oxidizing translation conditions. This report shows that the comp...

Cellulose sulfuric acid as a bio-polymer based solid catalyst efficiently catalyzes the selectively oxidation of sulfides to sulfoxides and thiols to disulfides using hydrogen peroxide as a green oxidant with good yields at room temperature. The developed method offers a number of advantages such as high selectivity, mild reaction conditions, simple operation, cleaner reaction profiles, low...

Hepatitis C virus glycoprotein E2 contains 18 conserved cysteines predicted to form nine disulfide pairs. In this study, a comprehensive cysteine-alanine mutagenesis scan of all 18 cysteine residues was performed in E1E2-pseudotyped retroviruses (HCVpp) and recombinant E2 receptor-binding domain (E2 residues 384 to 661 [E2(661)]). All 18 cysteine residues were absolutely required for HCVpp entr...