A reliable in vitro propagation protocol has been established from nodal segments of a highly valuable medicinal plant Solidago virgaurea L. The explants were cultured on Murashige and Skoog’s medium augmented with different concentrations and combinations of plant growth regulators for shoot bud initiation and multiplications. Highest frequency of shoot proliferation (91.8 ± 0.64) and maximum ...

An efficient in vitro plant regeneration protocol was developed for Chrysanthemum morifolium, an ornamental plant. Nodal segments were used as explants in the present experiment. The explants were cultured onto Murashige & Skoog’s (MS) nutrient medium containing plant growth regulators (cytokinins or auxins) with various combinations and concentrations for the study. Maximum percentage (93.33%)...

A rapid and efficient protocol was developed for inducing indirect organogenesis using leaf explants of Vitex negundo L. Explants were cultured on MS with different concentrations of 2,4-D and IAA in combination with BAP for callus induction. The frequency of callus induction increased with increasing concentration of IAA (0.3 mg/l) and BAP (0.3 mg/l) at optimal level. The shoot buds appeared e...

Polyploid plantlets, including triploid, tetraploid, and mixoploid, were induced from the European pear (Pyrus communis L.) cultivar ‘Fertility’ by in vitro colchicine treatment of leaf explants. The leaf explants were incubated in 0.4% (w/v) colchicine for 24, 48, or 72 h, then transferred to adventitious shoot-induction medium. Regenerated shoots were pre-selected according to their morpholog...

A simple micropropagation method from shoot tip explants was reported here for Clitoria ternatea L., an economically important medicinal plant. High frequency of micro shoots were obtained from this explants on MS medium supplemented with various concentrations of Indole Acetic acid (IAA) (0.5 2.0 mg/l) and KN (0.5 -2.0 mg/l). Maximum number of shoots was obtained from shoot tip explants in the...

The frequency of shoot regeneration and multiplication of P. harmala was influenced by the type of explant and kind and concentration of hormones. Of the various seedling explants, cotyledonary node exhibited maximum shoot regeneration frequency from axillary region on MS medium supplemented with 5 microM BAP. Addition of 0.1 microM NAA enhanced the efficacy of BAP for multiple shoot regenerati...

Eclipta alba is a medicinal herb, also known as Bhringraj belonging to family Asteraceae. The plant has hepatoprotective, antimicrobial and hair growth promoting properties. The effect of various phytohormones on in-vitro callus induction and shoot regeneration in Eclipta alba from leaf explant was evaluated. The sterilized explants were inoculated on MS medium supplemented with different conce...

A novel combination of plant growth regulators comprising indole-3-butyric acid (IBA), 6-benzylaminopurine (BA) and gibberellic acid (GA3) in Murashige and Skoog basal medium has been formulated for in vitro induction of both shoot and root in one culture using cotyledonary node explants of guar, (Cyamopsis tetragonoloba). Highest percentages of shoot (92%) and root (80%) induction were obtaine...

background & aim: dracocephalum genus belongs to the lamiaceae family which has eight species in iran. dracocephalum kotschyi boiss is an important medicinal and aromatic plant that use in pharmaceutical industry. in addition, this species is used in iranian traditional medicine. the aim of current study was to examine the various growth regulations and explants on direct organogenesis of d...

An effective and improved plant regeneration system was successfully developed using shoot tip explants taken from a two years old mature plant of Cassia angustifolia. The effect of different cytokinins, [6-benzyladenine (BA), Kinetin (Kin) and thidiazuron (TDZ)] at different concentrations (0.5-10 μM) were evaluated as augmented with Murashige and Skoog (MS 1962) medium. Among all the cytokini...