Homogenates prepared from the livers of rats treated for 3 days with the uncoupling agent 2,4-dinitrophenol contained considerably higher cyanide-sensitive superoxide dismutase activity when compared with homogenates of saline-treated animals. The effect was specific for only one form of the enzyme, however. Administration of 2,4-dinitrophenol at a dosage and on a schedule which produced the cl...

Ethanol stimulated the uptake of l-alanine into isolated membrane vesicles of a marine pseudomonad at a rate and to an extent comparable with that obtained with reduced nicotinamide adenine dinucleotide (NADH) or the artificial electron donor ascorbate-N, N, N', N'-tetramethyl-p-phenylenediamine (ascorbate-TMPD). Methanol and branched-chain alcohols had little or no capacity to energize transpo...

muscle, which is particularly great with DNP. Further experiments on these problems are in progress. SUMMARY 1. The influence of 2:4-dinitrophenol, methylene blue, brilliant cresyl blue, thionine, phenosafranine and Janus Green B on the oxidative phosphoryl-ation of mitochondria isolated from liver, kidney, brain, heart and skeletal muscle of rats has been studied in vitro as well as in vivo. 2...

A series of experiments was performed to test the proposal of others that the oxidation of ethanol to acetate is NADflinked, and that, when transferred into the mitochondria, the cytoplasmic reducing equivalents produced during ethanol oxidation inhibit the oxidation of glucogenic precursors required for the formation of P-enolpyruvate. Particulate-free dialyzed extracts of rabbit livers oxidiz...

Beck, Jay V. (Brigham Young University, Provo, Utah), and Fred M. Shafia. Effect of phosphate ion and 2,4-dinitrophenol on the activity of cell suspensions of Thiobacillus ferrooxidans. J. Bacteriol. 88:850-857. 1964.-The rate of oxidation of ferrous salts or elemental sulfur by aged cell suspensions, phosphate-depleted cells, or 2,4-dinitrophenol (DNP)-treated cells of Thiobacillus ferrooxidan...