نتایج جستجو برای: digestion method
تعداد نتایج: 1660798 فیلتر نتایج به سال:
The current study is to develop a gentle and efficient method for purification of fibroblast-activation protein positive (FAP+) cancer-associated fibroblasts (CAFs) from tumor tissues. Fresh tissues were isolated from BALB/c-Nude mice bearing human liver cancer cell line (HepG2), fully minced and separated into three parts, and digested with trypsin digestion and then treated with collagenase t...
Background and Objective: Hydrolysis of fat, oil and grease by ultrasonic waves is a pre-treatment method before anaerobic digestion which can change their physical, chemical and biological properties. The main purpose of this study was to investigate the efficiency of ultrasonic waves to improve the hydrolysis process and its use as an auxiliary substrate to increase the efficiency of anaerobi...
background: the clinical diagnosis of meningitis is crucial, particularly in children. the early diagnosis and empiric antibiotic treatments have led to a reduction in morbidity and mortality rates. pcr and the enzymatic digestion of 16srdna fragment which is produced by universal primers led up fast and sensitive determination. the purpose of this study was to investigate a rapid method fo...
Molecular detection of pathogenic microorganisms in ticks is based on DNA amplification of the target pathogen; therefore, extraction of DNA from the tick is a major step. In this study, we compared three different tick DNA extraction protocols based on an enzymatic digestion by proteinase K followed by DNA extraction by a commercial kit (method 1), or on mortar crushing, proteinase K digestion...
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