Abstract Introduction Canine leptospirosis has always been a differential diagnosis in dogs presenting with clinical signs and blood profiles associated kidney and/or liver disease. The conventional polymerase chain reaction (PCR) provides diagnoses, but real-time PCR-based tests provide earlier confirmation determine the severity of infection, especially acute stage, allowing early detection f...

Abbreviations: ARP: acidic ribosomal protein CHS: chalcone synthase DDRT-PCR: differential display reverse transcriptase polymerase chain reaction DETs: differentially expressed transcripts DPA: days post anthesis ESTs: expressed sequence tags MBD: methyl CpG binding domain OPT: oligopeptide transporter protein PCR: polymerase chain reaction PG: polygalacturonase enzyme RRF: ribosome recycling ...

introduction : frosted branch angiitis(fba) is a panuveitis with sheating of all retinal vesssels. case presentation : herein we report an immunocompetent person who presented with fever, headache, atypical rash, and hazy vision. ophthalmoscopy of both eyes revealed perivascular sheathing with frosted branch angiitis pattern in veins, patchy retinal hemorrhages. aqueous pcr analysis turned posi...

DNA fragments that show a pattern of differential expression on differential display gels must be eluted from the gel matrix and reamplified to enable further analysis. Elution is usually achieved by heating excised gel slices in a small volume of either water or TE. Here we show that this elution step can adversely affect the ability of the eluted DNA to act as a template for PCR reamplificati...

The majority of immunological processes are mediated by cell-to-cell contact or receptor-ligand interactions that transmit intracellular signals and affect the regulation of transcription in the nucleus. As a consequence, precursor cells develop into their respective lineages and cells differentiate further during an immune response. In order to study changes in normal cells or even cells that ...

Canine babesiosis is a tickborne, protozoal, haemoparasitic disease. Babesia organisms are frequently classified as either large (B. canis) or small gibsoni). The aim of this study was an attempt to detect B. gibsoni DNA in blood samples taken from dogs suspected suffering tick-borne diseases. 216 were tested using PCR, which, 99 them canisDNA detected, whereas 3 detected. Positive PCR results ...