Objective: In this study, toxic effects of Roundup, one the most common glyphosate-based herbicides (GBHs), were assessed on human bronchial epithelial cells (BEAS-2B).
 Material and Method: MTT neutral red uptake assays implemented for evaluation cell viability at 24 48 h. Apoptosis detection was made by Muse analyzer while Hoechst staining employed to detect apoptotic nuclear changes. ad...

The effect of chronic As exposure in brain, the specific oxidants production and oxidative stress still needs more attention. In present study, male wistar rats were chronically exposed to bythe drinking water during 60 days. receiving a dose 10 mg As/l showed 9- 18-fold increase content blood respectively, as compared control group. Histopathological changes detected brain samples isolated fro...

This study was investigated to evaluate the in vitro anti-diabetic activity and lipid accumulation inhibitory effect of domestic walnut (Junglans regia, Gimcheon 1ho cultivar) high glucose- oleic acid-induced hepatic HepG2 cells. Eighty percent ethanolic extract from (GC) showed higher effects against α-amylase α-glucosidase activities when compared other extracts. GC significantly inhibited fo...

This paper presents a detailed chemical and toxicological characterization of the diesel particulate matter (PM) emitted from vehicles running on chassis dynamometer under different driving conditions. Chemical analyses were performed to characterize contents organic carbon (OC), elemental (EC), 31 polycyclic aromatic hydrocarbons (PAHs) in collected PM samples. The OC–EC analysis results revea...

We have been developing the use of plasma-membrane-bound fluorescent probes to measure the pH values at the surfaces of living chondrocytes. For this purpose, three lipophilic pH indicators were made by covalently binding the xanthene dyes fluorescein, eosin or dichlorofluorescein to the amino group of phosphatidylethanolamine. The probes were incorporated into phospholipid vesicles and the eff...

Glycyrrhiza uralensis (or licorice) is a widely used Oriental herbal medicine from which the phenylflavonoids dehydroglyasperin C (DGC), dehydroglyasperin D (DGD), and isoangustone A (IsoA) are derived. The purpose of the present study was to evaluate the antioxidant properties of DGC, DGD, and IsoA. The three compounds showed strong ferric reducing activities and effectively scavenged DPPH, AB...

The inherent biochemical, anatomical and physiological characteristics of the brain make it especially vulnerable to insult. Specifically, some of these characteristics such as myelin and a high energy requirement provide for the introduction of free radical-induced insult. Recently, the biochemistry of free radicals has received considerable attention. It also has become increasingly apparent ...

Dichlorofluorescein (DCFH) oxidation assay measures hydrogen peroxide (H2O2), which is a derivative of superoxide anion. We found that a calmodulin antagonist, W-13, which is known to inhibit superoxide anion generation enhanced the capacity of human neutrophils to oxidize DCFH. To investigate this discrepancy we studied the role of nitric oxide (NO) in DCFH oxidation. Pure NO was capable of ox...

In this study, a new method for detecting creatinine was developed. This novel sensor comprised of two ionic liquids, poly-lactic-co-glycolic acid (PLGA) and 1-butyl-3-methylimidazolium (BMIM) chloride, in the presence of 2',7'-dichlorofluorescein diacetate (DCFH-DA). PLGA and BMIM chloride formed a functional porous polymer structure (FPPS)-like structure. Creatinine within the FPPS rapidly hy...

The influence of spherical nanoparticles (NPs) of nickel Ni° (70±0.3 nm in diameter) in the concentrations of 0.025, 0.05 and 0.1 M on the root part of 4-day-old seedlings of wheat Triticum vulgare wheat has been studied. After treating plants with nanoparticles, an increase was observed in the fluorescence of dichlorofluorescein and malondialdehyde (MDA), as well as strong haphazard DNA degrad...