The clinical picture secondary to amanita phalloides, which began with gastrointestinal complaints, advanced to fulminant hepatic failure in two days. Emergency liver transplantation was decided for the case of a 48-year-old male patient, who at the same time had renal failure and acute pancreatitis. Bridge treatment with plasma diafiltration was applied until the liver transplantation, which w...

DESCRIPTION Tripedia , Diphtheria and Tetanus Toxoids and Acellular Pertussis Vaccine Adsorbed (DTaP), for intramuscular use, is a sterile preparation of diphtheria and tetanus toxoids adsorbed, with acellular pertussis vaccine in an isotonic sodium chloride solution containing thimerosal as a preservative and sodium phosphate to control pH. After shaking, the vaccine is a homogeneous white sus...

The quickly developing field of "click" chemistry would undoubtedly benefit from the availability of an easy and efficient technology for product purification to reduce the potential health risks associated with the presence of copper in the final product. Therefore, solvent-resistant nanofiltration (SRNF) membranes have been developed to selectively separate "clicked" polymers from the copper ...

Microfiltration and diafiltration were conducted to obtain 3 distinct levels of liquid micellar casein concentrates from raw skim milk: 8, 10 11.5 g casein/100 g. All the subjected 300, 450 600 MPa for 5 min. The physicochemical microbiological changes in matrix studied compared with their untreated counterparts on day production after storing samples 7 d at °C. Slight pH correlated pressurizat...

Filtration steps are ubiquitous in biotech processes due to the simplicity of operation, ease of scalability and the myriad of operations that they can be used for. Microfiltration, depth filtration, ultrafiltration and diafiltration are some of the most commonly used biotech unit operations. For clean feed streams, when fouling is minimal, scaling of these unit operations is performed linearly...

The Labscale tangential flow diafiltration system in conjunction with polyethersulfone Biomax filters (both purchased from Millipore Inc.) were used in the following studies: (1) A crucial step in the preparation of polyhemoglobin (polyHb) is the removal of tetrameric hemoglobin (Hb), which can cause adverse side-effects. The efficiency for this depends on the integrity of the 100kDa filters. T...

The goal of the present work was to optimise the different environmental friendly processes involved in the extraction and purification of chondroitin sulphate (CS) from Prionace glauca head wastes. The experimental development was based on second order rotatable designs and evaluated by response surface methodology combined with a previous kinetic approach. The sequential stages optimised were...

This application note demonstrates the performance of Capto Core 700 chromatography medium (resin) as an alternative for ovalbumin removal in the production of egg-based influenza vaccine. Ovalbumin removal and haemagglutinin (HA) recovery were determined after the Capto Core 700 step in combination with ultrafiltration and diafiltration (UF/DF). The process performance was compared with that o...

Abstract Background Porcine Parvovirus (PPV) is a Parvovirinae virus that can cause embryonic and fetal loss death mummification in affected pigs. Unlike conventional vaccines, virus-like particles (VLPs) inherit the natural structure of their authentic virions highly immunostimulatory induce strong humoral immune T cell responses with no risk pathogenicity. The production PPV VLPs still challe...