We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent Ca(2+) indicator that can be covalently linked to SNAP-tag fusion proteins and retains its Ca(2+) sensing ability after coupling to protein.

In the educational context it is examined the teacher-stress association. The teacher’s stress is related with variables of academic environment, of out of the school and of the teacher. The impact of that association can be see in the teacher’s health, in his achievement and in the quality of the teaching-learning process. With the objective to present one view of how issue is being considered...

We demonstrate that an oft-used indirect attitude assessment technique—the attitude activation paradigm—accurately assesses attitudes only when participants attend to the prime stimuli during the attitude activation task. Attitude activation attitudes toward obviously valenced words (e.g., torture, liberty) were more sensitive to attitude valence and extremity when participants were required to...

In 2006, Daim et al. published the highly cited paper on forecasting emerging technologies with bibliometrics and patent analysis. In the paper, scientific publications and patent were used as a numerical input to for example system dynamic models or scenarios –elaborating on the current state and trend of technological development as a year-to-year indicator value. By forcing the indicator to ...

Background and Objectives: Data on patient complains can be used as a feedback to improve the quality of healthcare services. The aim of this study was to develop an indicator for reliable retrieval of statistical data on patient complaints.   Methods: A triangulated study was conducted. A comprehensive literature review was done to identifying the relevant major themes. Two-rounds of Delphi ...

This paper presents some aspects related to the issues of aggregating economic indicators. Departing from the research of Gh. Paun (1983) we will prove a theorem which states that under certain, natural assumptions, it is impossible to obtain an optimum aggregation. Unlike the original work of Paun, in this paper we are giving a rather simple proof of this theorem.

The validity of the Michaelis-Menten-Briggs-Haldane approximation for single enzyme reactions has recently been improved by the formalism of the total quasi-steady-state approximation. This approach is here extended to fully competitive systems, and a criterion for its validity is provided. We show that it extends the Michaelis-Menten-Briggs-Haldane approximation for such systems for a wide ran...