Dentine hypersensitivity or more colloquially “sensitive teeth” is a common problem encountered in routine clinical practice. The diagnosis of it, at times, is intriguing for even the most experienced clinician. The condition, though not alarming, is important diagnostically and therapeutically because it tends to disturb the routine day to day life of the patient, often becoming troublesome du...

Laser irradiation during root canal treatment is efficient but in order to prevent irreversible thermal damage to dentine, cementum and other periapical structures continuous water cooling is required. The aim of this study was to ultrastructurally analyze the surface of intracanal dentine and cementum at the root apex after Er:YAG laser irradiation with and without sterile saline solution irri...

Root caries prevention has been a challenge for clinicians due to its special anatomical location, which favors the accumulation of dental plaque. Researchers are looking for anti-biofouling material to inhibit bacterial growth on exposed root surfaces. This study aimed to develop polydopamine-induced-polyethylene glycol (PEG) and to study its anti-biofouling effect against a multi-species cari...

Currently, it is still a tough task for dentists to remineralize dentine in deep caries. The aim of this study was to remineralize demineralized dentine in a tooth model of deep caries using nanocomplexes of carboxymethyl chitosan/amorphous calcium phosphate (CMC/ACP) based on mimicking the stabilizing effect of dentine matrix protein 1 (DMP1) on ACP in the biomineralization of dentine. The exp...

Aim: This study determined whether prior brushing with desensitizing toothpastes (Sensodyne Rapid Relief and Colgate Pro-Relief) affected the shear bond strengths of composite to dentine surfaces after appropriate use of two different dentine bonding agents (SingleBond and PQ-1). Materials and Methods: Sixty caries free molar teeth were cleaned, disinfected and embedded in individual cylinders ...

OBJECTIVE To investigate and compare the protective impact of the in situ formed salivary pellicle on enamel and dentine erosion caused by different acids at pH 2.6. METHODS Bovine enamel and dentine samples were exposed for 120 min in the oral cavity of 10 healthy volunteers. Subsequently, enamel and dentine pellicle-covered specimens were extraorally immersed in 1 ml hydrochloric, citric or...

Aim of this study was to investigate the distribution of versican proteoglycan within the human dentine organic matrix by means of a correlative immunohistochemical analysis with field emission in-lens scanning electron microscope (FEI-SEM), transmission electron microscope (TEM), fluorescence microscope (FM) and biochemical assay. Specimens containing dentine and predentine were obtained from ...

A dentine protein extraction protocol was modified in order to identify matrix metalloproteinase gelatinolytic activities in the non-mineralized and mineralized phases of human dentine. Dentine proteins from 24 individual permanent molars from patients aged 15-73 years were sequentially extracted, first with guanidinium chloride (G1 extract), then EDTA (E extract), and after this demineralizati...

OBJECTIVE The aim of the present study is to investigate the ultrastructural changes within the different zones of carious dentine and compare those changes with sound dentine. METHODS Transmission electron microscopy and electron diffraction techniques were used to investigate the effect of caries on the inorganic phase of dentine. Areas of interest were identified with optical and scanning ...

This in vitro study evaluated the effect of commercially available remineralising products on demineralised dentine. Demineralised dentine was stored in pooled saliva or treated with Tooth Mousse, Tooth Mousse Plus, Gel-Kam or NeutraFluor 5000, for 7 days at room temperature. Dentine slices were microhardness tested, and assessed using scanning electron microscopy (SEM) and energy dispersive an...