The medullary thick ascending limb (MTAL) reabsorbs solute without water and concentrates [Formula: see text] in the interstitium without a favorable pH gradient, activities which require low water and NH3 permeabilities. The contributions of different apical and basolateral membrane structures to these low permeabilities are unclear. We isolated highly purified apical and basolateral MTAL plas...

To evaluate the effect of a dentin adhesive on sclerotic dentin, contraction gap width and shear bond strength were measured. Dentin cavity wall was pretreated with an experimental dentin bonding system with and without a dentin primer, or with a commercial dentin bonding system. In the experimental dentin bonding groups, contraction gap width of sclerotic dentin was significantly smaller than ...

The number and the mean percentage tubular cross-sectional area of dentinal tubules per square millimetre were calculated in specimens of coronal dentine of 13 intact human third molar teeth from patients 18 to 28 years of age. The dentine was fractured at various known distances from the dentino-enamel junction. Near the dentino-enamel junction the number of tubules per square millimetre was 2...

The aim of this study was to evaluate the microtensile bond strength (microTBS) and the elemental contents of the adhesive interface created to normal versus caries-affected dentin. Extracted human molars with coronal carious lesions were used in this study. A self-etching primer/adhesive system (Clearfil Protect Bond) was applied to flat dentin surfaces with normal and caries-affected dentin a...

The diffusion through dentine by a number of biological and synthetic molecules, including resins and dyes, is reported. In vitro measurements were derived by experiments with a modified 'split-chamber device'. Diffusion was found to be indirectly proportional to dentine thickness for all molecules. Permeability of water-soluble molecules and ethanol-soluble molecules was proportional to the mo...

The relation between the bonding efficacy of the dentin adhesive and the physical change of the dentin after treatment with various dentin cleansers was investigated. The dentin surface was cleaned with one of the seven experimental dentin cleansers, 5, 10, 20, 30 and 40% of the phosphoric acid, neutralized 0.5 M EDTA solution (pH 7.4) and 9.1% pyruvic acid containing 9.1% glycine, prior to the...

This study evaluated the effect of dentin surface treatment on shear bond strengths of two light- cured glass ionomer restorative materials to dentin. Cylinders of Fuji II LC and VariGlass VLC were bonded to dentin surfaces that were untreated, treated with a 10% polyacrylic acid (GC Conditioner), or treated with a dentin bonding agent primer (Prisma Universal Bond 3 Primer). Specimens were the...