T HE SEPARATION of morphologically distinct cells by differences in their specific gravity has been demonstrated using density gradient centrifugation in several support media. From bovine serum albumin density gradient centrifugation experiments, morphologically similar lymphocytes have been shown to consist of several closely spaced, discontinuous, discrete populations.5 In addition, erythroc...

background: it has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. these methods also reduce sperm chromatin deficiencies and screen the sperms before applying in assisted reproduction techniques. objective: this study compared simple density gradient methods and a combin...

A method of evaluating the iodine distribution in thryroglobulin has been developed. It is based on the increase in density that occurs when iodine is incorporated into proteins. Resolution of iodinated species is obtained by equilibrium centrifugation in a 34.5% (w/w) RbCl density gradient (isopycnic centrifugation) in either the analytical or preparative ultracentrifuge. The equilibrium in 34...

This report describes a 200- to 300-fold increase in the quantity of ribonucleic acud tumor virus particles previously isolated at one time by zonal centrifugation.

Bone marrow mononuclear cells (BMNCs) are widely used in regenerative medicine, but recent data suggests that the isolation of BMNCs by commonly used Ficoll-Paque density gradient centrifugation (DGC) causes significant cell loss and influences graft function. The objective of this study was to determine in an animal study whether and how Ficoll-Paque DGC affects the yield and composition of BM...

The osmolarity and density of colloids used to prepare spermatozoa for assisted reproduction may affect sperm quality in the resultant preparation. In this study, two osmolarities of Androcoll-E for single-layer or density gradient centrifugation of stallion spermatozoa were compared: "normal" (320 mOsm) or "high" (345 mOsm). Mean yields for the two centrifugation techniques did not differ betw...

The Artificial Insemination (AI) is the first option treatment for infertile couples with cervical factor subfertility, mild-moderate male subfertility and unexplained infertility. With the exception of cases in which the use of in vitro fertilization (IVF or ICSI) is strictly due as a consequence of a severe male or female factor, the artificial insemination must be part of a gradual approach ...

A method is described for the rapid preparation of peroxisomes from rat liver by using sucrose-density-gradient centrifugation in a vertical rotor. The preparation, shown to be virtually free of mitochondrial and microsomal contamination, can be used to study fatty acid metabolism by isolated peroxisomes.

Artificial insemination using sexing semen is expected to produce calves with the desired sex. One sperm method percoll density gradient centrifugation method. This study aimed determine changes and relationship between motility acrosome reaction after process centrifugation. The material used was of ±5 years old Ongole crossbred bull a bodyweight ±700 kg as many three bulls mass 2+ individual ...