Two novel cytotoxic and antifungal constituents, (4S,6S)-6-[(1S,2R)-1, 2-dihydroxybutyl]-4-hydroxy-4-methoxytetrahydro-2H-pyran-2-one (1), (6S,2E)-6-hydroxy-3-methoxy-5-oxodec-2-enoic acid (2), together with three known compounds, LL-P880γ (3), LL-P880α (4), and Ergosta-5,7,22-trien-3b-ol (5) were isolated from the metabolites of endophytic fungi from Dendrobium officinale. The chemical structu...

A critical step in the development of Agrobacterium tumefaciens-mediated transformation in orchid genus is the establishment of optimal conditions for T-DNA gene delivery into protocorm-like bodies from which the whole plantlets can be regenerated. As a first step in the development of a successful Agrobacterium tumefaciens-mediated transformation for Dendrobium savin white, factors influencing...

An experiment was carried out in Plant Tissue Culture Laboratory, Department of Crop Botany, Bangladesh Agricultural University, Mymensingh to investigate the effect of banana extract on micropropagation of Dendrobium sp. var. Sonia orchid through PLBs. The experiment was conducted during July 2012 to October 2013. Half-Murashige and Skoog (1/2MS) medium were used as basal medium and the medium...

Molecular systematic research, using DNA sequences of the internal transcribed spacer (ITS) region of the 18-26S nuclear ribosomal repeat unit, was conducted on a broadly representative sample of the tribe Dendrobieae. The results provide independent support, in addition to evidence from plastid DNA analysis and morphology, for the phylogenetic reassessment of the taxon. At a broad level, Dendr...

An experiment was conducted to determine the influence of concentration and mode of application of different growth regulators on growth and yield of Dendrobium hybrid “Thongchai Gold” in subtropical cool climate zone. Various concentrations of GA 3 and BA were applied as foliar spray and drenching mode. GA 100 ppm in combination with BA 100 ppm followed by GA 200 ppm 3 3 increased the plant he...

During speciation in flowering plants, many morphological characters were evolved in response to continuous selection pressure. Such characters are largely governed by genetic changes or get modulated according to ecological adaptations. To explore the potential of latter, a novel comparative analysis of seed micromorphology and ultrastructure was performed in twenty species in the genus Dendro...

Nuntipa KHUMKARJORN, Sudarat THANONKEO, Mamoru YAMADA, Preekamol KLANRIT, Pornthap THANONKEO* Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, Thailand Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan Department of Bi...

Sensitive, selective and reliable high-performance liquid chromatography (HPLC)-diode array detection (DAD)/electrospray ionization multi-stage mass spectrometry (ESI-MSn) methods have been developed for the characterization of nine 2-glucosyloxycinnamic acid derivatives and quantitative analysis of three of the major 2-glucosyloxycinnamic acids, cis-melilotoside, trans-melilotoside and dihydro...

We studied the effect of precooling and the use of an ethylene absorbent (based on potassium permanganate) in the flower boxes, on the vase life of Dendrobium ‘Pompadour’ flowers, after simulation of air shipment (3 days at 25°C). Precooling at 10°C (85-95%RH) for 60 minutes reduced ethylene production, ACC activity, and the concentration of 1-aminocyclopropane-1-carboxylic acid (ACC) in the fl...