This paper examines the influence of acylation of the Lys-ε-NH(3)(+) groups of bovine carbonic anhydrase (BCA, EC 4.2.1.1) to Lys-ε-NHCOR (R = -CH(3), -CH(2)CH(3), and -CH(CH(3))(2), -CF(3)) on the rate of denaturation of this protein in buffer containing sodium dodecyl sulfate (SDS). Analysis of the rates suggested separate effects due to electrostatic charge and hydrophobic interactions. Rate...

Modal DNA (ploidy) and sensitivity of DNA in situ to denaturation by acid have been analyzed by flow cytometry of 10 colorectal adenomas and 35 adenocarcinomas; 39 normal mucosa samples served as controls. A new method was developed to denature DNA in chromatin of the freshly isolated, intact, and unfixed individual cell nuclei from surgically resected material. The sensitivity of DNA denaturat...

Inflammation is a normal protective reaction against tissue damage caused by physical injury, harmful chemicals, and protein denaturation. Protein denaturation process in which proteins lose their tertiary structure secondary due to foreign substances, external compounds, such as strong acids, bases, organic salts, solvents, heating. The purpose of this study was determine the anti-inflammatory...

Lactoferrin (LF) is a multifunctional protein in the transferrin family that has been widely used food and pharmaceutical products. However, it susceptible to denaturation during thermal processing, which can diminish functionality bioavailability of LF final product. Coacervation with biopolymers demonstrated as promising approach protect from denaturation. This work aims study formation condi...

Green fluorescent protein (GFP) is an unusually stable autofluorescent protein that is increasingly being exploited for many applications. In this report, we have used fluorescence spectroscopy to study the effect of pH on the denaturation of GFP with sodium dodecyl sulfate (SDS), urea, and heat. Surprisingly, SDS (up to 0.5%) did not have any significant effect on the fluorescence of GFP at pH...

The Transfer-Integral (TI) operator is a powerful method to investigate the statistical physics of 1-dimensional models, like those used describe DNA denaturation. At cost certain number approximations, TI equation can be reduced Pseudo-Schr\"odinger Equation (PSE), according which sequence equivalent point particle moving in potential well. In this paper, I check validity standard PSE approxim...

The kinetic properties and thermal characteristics of fresh pork meat proteins (Longissimus dorsi), as well osmotically dehydrated proteins, were investigated using differential scanning calorimetry. Two isoconversional kinetical methods, namely the Friedman integral Ortega employed to analyze data. obtained triplet, activation energy, pre-exponential factor, extent conversion, has been discuss...

The effect of heterogeneous sequence composition on the denaturation of double stranded DNA is investigated. The resulting pair-binding energy variation is found to have a negligible effect on the critical properties of the smooth second order melting transition in the simplest (Peyrard-Bishop) model. However, sequence heterogeneity is dramatically amplified upon adopting a more realistic treat...