The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and r...

For several decades, people have wondered why pregnant mothers do not reject fetuses bearing allogeneic paternal antigens. Several hypotheses have been proposed, including a physical barrier between fetus and mother, immaturity of fetal antigens and temporary dormancy of the maternal immune system. Based on the "cell death immune recognition model," the author proposes a hypothesis that pregnan...

Lactate dehydrogenase C, an isoenzyme composed of C polypeptide subunits and found only in mature testes and spermatozoa, differs kinetically, chemically and immunologically from the five common isoenzymes of lactate dehydrogenase, each of which is a tetramer of A and/or B subunits. In the rat lactate dehydrogenase C exists in two molecular forms, isoenzymes C4 and A1C3. In addition to these tw...

BACKGROUND Reactive oxygen species (ROS)-mediated damage to human spermatozoa has been implicated in the pathogenesis of male infertility. Although ROS production by human spermatozoa has been extensively studied, the cell-to-cell variation in ROS production by spermatozoa at different stages of maturation has never been investigated. METHODS In this study, we determined ROS production by sub...

background: sodium arsenite can impair male reproductive function by inducing oxidative stress. silymarin is known as a potent antioxidant.objective: this study was performed to investigate if silymarin can prevent the adverse effect of sodium arsenite on ram sperm viability, motility and mitochondrial membrane potential.materials and methods: epidydimal spermatozoa obtained from ram were divid...

Cryopreservation is a technique for storing cells and tissues at very low temperatures the possible usage of stored throughout year. Sperm cell cryopreservation in some species causes decrease sperm quality DNA damage. Inappropriate protocols can cause changes physiology. Mitochondria are organelles that play role producing energy motility. have molecules with small sizes compared to structures...

Testicular and epididymal spermatozoa are routinely used with in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) to achieve pregnancies. In addition, excess cryopreserved spermatozoa can be thawed and used for ICSI. However, information on the recovery of epididymal and testicular spermatozoa after freeze-thaw is lacking. This is important to determine the feasibility of u...

BACKGROUND Reactive oxygen species (ROS)-induced damage of membrane phospholipids and DNA in human spermatozoa has been implicated in the pathogenesis of male infertility. In this study, variations in ROS production, DNA structure (as measured by the sperm chromatin structure assay) and lipid composition, were studied in human spermatozoa at different stages of maturation. METHODS Sperm subse...

The aim of the study was to examine sperm viability of Pinzgau bull insemination doses following longor short-term storage. Insemination doses, provided by Slovak Biological Services Inc. (Lužianky, Slovak Republic), were slow-frozen and stored in containers with liquid nitrogen for 1 18 years. The sperm samples were divided, according to the length of storage, to the short-term ( ≤ 15 years) a...

Sperm motility is an important parameter for success in intracytoplasmic sperm injection (ICSI) treatment. The sperm motility is due to its tail. If the sperm is not moving, it could be dead or alive. Normal live births have been obtained after ICSI with immotile sperm [1,2]. Various procedures have been applied to pick viable sperm for ICSI including, hypo-osmotic swelling (HOS) test [3], addi...