Reproduction of the corn cyst nematode (Heterodera zeae) and its effect on growth of corn (Zea mays) was studied in plant growth chambers at 24, 27, 30, 33, and 36 C. Reproduction of H. zeae increased directly with increase in temperature from 24 to 36 C. Fifteen-cm-d pots of corn seedlings inoculated with a mixture of 5,000 eggs + J2 and maintained for 8 weeks in growth chambers contained an a...

Like any other branch of Agricultural Sciences, the Division of Nematology at IARI, initiated researches in the exciting field of biochemistry and molecular biology of plant parasitic nematodes during the early seventies of last century. The main research areas were biochemical mechanism of resistance against phytonematodes and diagnostics of economically important plant parasitic nematodes. Sp...

1) Effects of cereal cyst (Heterodera) and rootlesion (Pratylenchus) nematodes on wheat are difficult to identify and control. Symptoms are non-specific and easily confused with stress from nutrient deficiency, drought, or disease. 2) Multiple species of Heterodera and Pratylenchus are capable of damaging wheat. Identification of species is difficult and procedures based on comparative morpholo...

To understand the machinery underlying a tomato cultivar harboring the Hero A gene against cyst nematode using microarrays, we first analyzed tomato gene expression in response to potato cyst nematode (PCN; Globodera rostochiensis) during the early incompatible and compatible interactions at 3 and 7 days post-inoculation (dpi). Transcript levels of the phenylalanine ammonia lyase (PAL) and Myb-...

Sasanelli, N., Vovlas, N., Trisciuzzi, N., Cantalapiedra-Navarrete, C., Palomares-Rius, J. E., and Castillo, P. 2013. Pathogenicity and host–parasite relationships of Heterodera cruciferae in cabbage. Plant Dis. 97:333-338. Stunted cabbage (‘Lupini’) associated with severe soil infestations by a cyst-forming nematode were observed in large patches of open fields in Castellaneta, province of Tar...

in this research, 30 sugar beet breeding populations developed by crossing of sugar beet monogerm and multigerm male strile lines with nematode resistant wild sources (w-1009 contaning resistance gene from beta procumbens and w-1010 contaning resistance genes from beta maritima) were compared with resistant and susceptible checks with respect to nematode resistance and yield. the experiments we...

In the quest for plant regulatory sequences capable of driving nematode-triggered effector gene expression in feeding structures, we show that promoter tagging is a valuable tool. A large collection of transgenic Arabidopsis plants was generated. They were transformed with a beta-glucuronidase gene functioning as a promoter tag. Three T-DNA constructs, pGV1047, p delta gusBin19, and pMOG553, we...

Summary Selected strains of Pasteuria species from the endospore-forming Gram-positive bacteria group have potential to be developed into control agents for plant-parasitic nematodes. If is deployed successfully as a agent, endospores bacterium initially adhere cuticle infective juvenile. Studies isolated root-knot nematodes suggested that collagen-like fibres on endospore surface interact with...

Soybean cyst nematode (SCN) is one of the most important diseases in soybean. Currently, main management strategy relies on planting resistant cultivars. However, overuse a single resistance source has led to selection virulent SCN populations, although mechanisms by which overcomes genes remain unknown. In this study, we used nematode-adapted single-cell RNA-seq approach identify potentially i...