The test is applied to substances, preparations, or articles METHOD which, according to the Pharmacopeia, are required to be sterile. However, a satisfactory result only indicates that no con1. Seed the indicator cell culture at a suitable density taminating microorganism has been found in the sample ex(for example, 2 × 104 to 2 × 105 cells/mL, 4 × 103 to amined under the conditions of the test...

Abstract Background and Objectives Proliferation and differentiation of mesenchymal stem cells (MSCs) in culture media requires the presence of nutrient supplements such as bovine fetal serum (FBS), which increases the risk of prion/xenotic infections. Platelet lysates as a rich source of growth factors and cytokines can replace FBS in cell culture medium. This study aimed to compare the effe...

The green colonial microalga, Botryococcus braunii is well known for its high lipid content and has already been proposed as a renewable energy source for various aquaculture and biotechnological applications. However, due to its slow growth rate compared with other microalgae, B. braunii has not yet been used in mass culture to produce more biomass. Therefore, in this study we tested different...

background: in order to avoid potential harm of synthetic additives food, development of novel functional foods containing natural ingredients is considered in current years. in this study, inhibitory effect of echinophora platyloba essential oil (eo) on growth of aspergillus flavus was evaluated in culture media and cheese. methods: e. platyloba eo was extracted by hydrodistillation method usi...

background: echinococcus granulosus cultivation is very important for improvement of different aspect of medical and veterinary researches. despite many advances in this case, there is a missing link for in vitro life cycle of adult worms and it is fertili-zation. regarding the researchers’ observations, self-fertilization can be done in worms living in dog intestine, but despite all sorts of e...

The test is applied to substances, preparations, or articles METHOD which, according to the Pharmacopeia, are required to be sterile. However, a satisfactory result only indicates that no con1. Seed the indicator cell culture at a suitable density taminating microorganism has been found in the sample ex(for example, 2 × 104 to 2 × 105 cells/mL, 4 × 103 to amined under the conditions of the test...

1. Human placental cord serum contains a factor or factors necessary for active growth of fibroblasts in tissue culture and a factor which prevents the appearance of a characteristic toxicity. 2. The growth-promoting factor(s) is stable to heating to 60 degrees C. for 30 minutes and is associated with the euglobulin fraction of the serum. 3. Extraction of dried cord serum with benzene, chlorofo...