نتایج جستجو برای: culture cell
تعداد نتایج: 1867614 فیلتر نتایج به سال:
The objective of this study was to evaluate the apoptotic effects of increasing granulosa cell density and increasing estradiol on relative gene expression associated with apoptosis factors (Bcl-2, Bax and GADD45β) and cell cycle (CCDN-2). For this reason after collecting ovaries of cows from an abattoir, granulosa cells were separated from 4- to 8-mm follicles. Then, viable cells were counted ...
background: several studies have demonstrated the immunosuppresive effects of mes-enchymal stem cells (mscs) in allogeneic or mitogenic interactions. cell-cell contact inhibition and secretion of suppressive soluble factors have been suggested in this re-gard. objective: to investigate if adipose derived mscs could inhibit jurkat lym-phoblastic leukemia t cell proliferation during coculture. me...
Background: Adipose tissue stem cells (ASCs) cause faster repair of damaged tissue posttransplantation by releasing growth factors in neurodegenerative diseases. ASCs secrete factors in the culture medium called conditioned medium (CM) in vitro. This study investigated the expression of neurotrophin genes in vitro culture and transplant of ASCs in Parkinsonian rats. Materials and Methods: In th...
Background: Noscapine is a water soluble alkaloid with antitussive property and very less toxicity. It is obtained from the Papaveraceae family. Recently, new pharmacological properties such as anticancer, anti stroke and anti-angiogenic effects are introduced. There is no scientific report on noscapine effect on reproductive system. The aim of present study was to determine in vitro effect of ...
تحقیقات مختلف اثبات نموده اند که رشد و محتوای بیوشیمیایی روتیفرها می تواند تحت تاثیر نوع غذا و غلظت مواد غذایی تغییر نماید. ریز جلبک ها به عنوان یکی از منابع غذایی مهم برای کشت روتیفرها به ویژه از لحاظ شاخص های نظیر رشد، بقا، میزان پروتئین، چربی و اسیدهای چرب، ضروری می باشند. لذا تحقیق حاضر جهت مقایسه جلبک خشک (کلرلای پودری) و جلبک های تازه (ایزوکرایسیس گالبانا و نانوکلروپسیس آکولاتا) بر رشد، ت...
هدف از انجام این مطالعه مقایسه دو روش mpn-pcr و mpn-culture در شمارش باکتری لیستریا مونوسیتوژنز تلقیح شده به شیر استریل می باشد. برای انجام این کار تعدادcell/ml 103 از باکتری لیستریا مونوسیتوژنز و نیز باکتری های زمینه ای مختلف به شیر استریل تلقیح شدند. پس از آماده سازی رقت های متوالی، از هر رقت در سه لوله حاوی محیط غنی کننده لیستریا تلقیح شد. پس از 48 ساعت گرمخانه گذاری، برای آزمایش mpn-cultu...
Introduction: peganum harmala L. (Zygophyllaceae) and varthemia persica DC (Compositeae) were used for co-culture studies. P.harmala was found to be one of the richest natural sources of β-carboline alkaloids. β-carboline alkaloids posses different neuropharmacologic activities including BDZ antagonism, MAOinhibitor and aminoreuptake inhibitor. The production of β-carboline alkaloids in cell ...
Introduction & Objective: Acanthamoeba is free-living amoeba that is found in soil, water, air as well as in human pharynx. Acanthamoeba is causative agent of granulomatose amoebic encephalitis (GAE) in immunosuppressed and AIDS individuals and amoebic keratitis in people who use the lens. Pathogenic species of Acanthamoeba have protein receptors named mannose binding protein (MBP). Acanthamo...
Background & Objective: Bone defects in jaws create major problems for oral and maxillofacial surgery. To overcome the limitations of Autografts tissue engineering uses autogenus cells and synthetic scaffolds. Type of cells or cell sources have an important effect on the construction which is produced. The aim of this study was to evaluate the feasibility and probable limitations of osteoblast...
allogeneic hematopoietic stem cell transplantation is used in the treatment of patients suffering from hematologic and non-hematologic disorders, but the application is limited by the identification of a suitable donor. umbilical cord blood (ucb) is an alternative source of hematopoietic stem cell (hsc) transplantation. despite all advantages, the limited cell dose is one of the major obstacle...
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