The gene coding for the 109-amino-acid, non-glycosylated form of mavicyanin was synthesized and expressed in Escherichia coli. The recombinant protein refolded from E. coli inclusion bodies was purified and characterized. Its spectroscopic properties are fully identical to those of mavicyanin isolated from zucchini, even in the absence of its carbohydrate moiety. The blue cooper center of mavic...

interspecific hybridization occurs among cucurbia species it is commonly used for grafting, yield increasing and production of pest resistant rootstocks. in the present research probability of interspecies hybrid generation among seven lines of cucurbita pepo (p1, p4, p5, p6, p10, p22, p25) and six lines of cucurbita moschata (mo3, mo5, mo6, mo9, mo11, mo12) were investigated. after crossing of...

The capacity of intact cells of roots excised from summer squash plants (Cucurbita pepo L. cv Early Prolific Straightneck) to synthesize purine nucleotides de novo was investigated. Evidence that purine nucleotides are synthesized de novo included: (a) demonstration of the incorporation of [1-(14)C]glycine, [2-(14)C]glycine, NaH(14)CO(3), and H(14)COONa into total adenine nucleotides; (b) obser...

The mitochondrial genomes of seed plants are unusually large and vary in size by at least an order of magnitude. Much of this variation occurs within a single family, the Cucurbitaceae, whose genomes range from an estimated 390 to 2,900 kb in size. We sequenced the mitochondrial genomes of Citrullus lanatus (watermelon: 379,236 nt) and Cucurbita pepo (zucchini: 982,833 nt)--the two smallest cha...

The Cucurbitaceae family accumulates hydrophobic organic pollutants in its aerial parts at high concentrations. Major latex-like proteins (MLPs) were identified zucchini ( Cucurbita pepo) as a transporting factor for pollutants. MLPs bind to the roots, are secreted xylem vessels complexes, and transported parts. However, suitable conditions binding remain elusive. In present study, we show that...