Combined coordination of Zn(II) and Cu(I) or Cu(II) to the amyloid-β peptide has been investigated using XANES, EPR and NMR spectroscopies. While Zn(II) does alter Cu(II) binding to Aβ, this has no effect on (Aβ)Cu induced ROS production and associated cell toxicity.

Paramagnetic Cu(II) ions enhance nuclear spin relaxation in a distance-dependent fashion and can be used as a structural probe of proteins. Cu(II) can also serve as a functionally important ligand in proteins. Here we investigate the structural basis of Cu(II) inhibition of the influenza M2 proton channel through Cu(II)-induced paramagnetic relaxation enhancement (PRE). (13)C T(1) relaxation ra...

Spectroscopic methods combined with density functional calculations are used to develop a detailed bonding description of the mu(4)-sulfide bridged tetranuclear Cu(Z) cluster in N(2)O reductase. The ground state of Cu(Z) has the 1Cu(II)/3Cu(I) configuration. The single electron hole dominantly resides on one Cu atom (Cu(I)) and partially delocalizes onto a second Cu atom (Cu(II)) via a Cu(I)-S-...

Reactions of the copper complexes Cu(II)Cl2, [Cu(II)(EDTA)]2-, [Cu(II)2(DIPS)4] and [Cu(I)(DMP)2]+ (where DIPS is 3,5-diisopropylsalicylate and DMP is 2,9-dimethylphenanthroline) with human blood plasma and urine have been studied by 500 MHz 1H NMR spectroscopy, and CD spectroscopy has been used to monitor the transfer of Cu(II) onto albumin in plasma. The rate of transfer of Cu(II) from [Cu(II...

Diglycyl-L-histidine is a peptide molecule designed to mimic the specific Cu(II) transport site of human albumin. The equilibria involved in the Cu(II)-diglycyl-L-histidine system have been investigated by analytical potentiometry in aqueous solution (0.15 M NaCl, 25’). The synthetic peptide molecule bound Cu(I1) exclusively as a 1: I complex in the pH range of 6.50 to 11.00. The results furthe...

Diglycyl-L-histidine is a peptide molecule designed to mimic the specific Cu(II) transport site of human albumin. The equilibria involved in the Cu(II)-diglycyl-L-histidine system have been investigated by analytical potentiometry in aqueous solution (0.15 M NaCl, 25’). The synthetic peptide molecule bound Cu(I1) exclusively as a 1: I complex in the pH range of 6.50 to 11.00. The results furthe...

The coupling of tetrazotised 1,8-diaminonaphthalene with 1,3-dicarbonyl compounds [acetylacetone,methylacetoacetate and acetoacetanilide] yielded a new series of tetradentate ligand systems. Analytical, IR,1H NMR and mass spectral data indicate that the compounds exist in the intramolecularly hydrogen bondeddihydrazone form. Dibasic tetradentate N2O2 coordination of these compounds in their [ML...

The Cu(II)- and Co(II)-binding properties of two peptides, designed on the basis of the active site sequence and structure of the blue copper protein plastocyanin, are explored. Peptide BCP-A, Ac-Trp-(Gly)(3)-Ser-Tyr-Cys-Ser-Pro-His-Gln-Gly-Ala-Gly-Met-(Gly )(3)-His-(Gly)(2)-Lys-CONH(2), conserves the Cu-binding loop of plastocyanin containing three of the four copper ligands and has a flexible...

A diverse set of guest molecules template the consistent formation of fifteen iso-structural layered structures containing a pyridine based tripodal ligand, divalent metal ions and anions. These materials have shown a remarkable ability to interconvert from Zn(II) to Cu(II), Cd(II) to Cu(II), Cu(II) to Cd(II) complexes in a SCSC (single crystal-to-single crystal) fashion.