نتایج جستجو برای: ct dna
تعداد نتایج: 639029 فیلتر نتایج به سال:
In this study the interaction of the complex with DNA have been investigated using UV/Vis , and thermal denaturation.The results showed that complex Ni(Pirox)2(DMF)2 can bind to ct-DNA. The binding constants of the complex with ct-DNA have also been calculated. The thermodynamic parameters for binding of complex with ct-DNA are also determined using van’t Hoff equation. Competitive binding with...
کمپلکسهای جدیدی از فلزات نیکل و مس(ii) که حاوی لیگاند دارویی مزالامین بود سنتز شد و با روش های طیف سنجی uv- vis، ft-ir، 1h nmr، طیف جرمی شناسایی شد. و بر هم کنش این دارو و کمپلکسهای آن با dna تیموس گوساله توسط روشهای اسپکتروفتومتری، فلوریمتری، دورنگ نمائی چرخشی، ویسکوزیمتری وهمچنین برهم کنش مزالامین با bsa توسط روشهای اسپکتروفتومتری، فلوریمتری، دورنگ نمائی دورانی بررسی گردید. کاهش در طیف جذبی د...
The interaction between isatin-β-thiosemicarbazone (IBT) and calf thymus DNA (CT-DNA) was investigated in physiological buffer (pH 7.4) using Neutral Red (NR) dye as a spectral probe by UV-Vis absorption and fluorescence spectroscopy, as well as viscosity measurements. The IBT is stabilized by intercalation in the DNA (K [IBT -DNA] = 1.03×10(5) M(-1)), and displaces the NR dye from the NR-DNA co...
background: dna methyltransferase-3b (dnmt3b) is an important enzyme responsible for maintaining the dna methylation pattern in eukaryotic cells. in this study we have investigated the correlation between the 46359c→t polymorphism in the dnmt3b gene and the risk of breast cancer incidence among sporadic breast cancer patients in fars province, southern iran. methods: in this case-control study,...
Photoexcited 2-aminopurine (Ap*) is extensively exploited as a fluorescent base analogue in the study of DNA structure and dynamics. Quenching of Ap* in DNA is often attributed to stacking interactions between Ap* and DNA bases, despite compelling evidence indicating that charge transfer (CT) between Ap* and DNA bases contributes to quenching. Here we present direct chemical evidence that Ap* u...
Background: DNA damage is one of the major consequences of radiation exposure onto the biological systems. A series of compounds including flavanoids were found to render DNA protection against radiation damage. In this study we elucidated the potential of rutin and rutin hydrate to protect plasmid DNA against damage induced by irradiation. Materials and Methods: DPPH and hydroxyl radical scave...
background: dna isolation procedure can significantly influence the quantification of dna by real time pcr specially when cell free dna (cfdna) is the subject. to assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfdna, development of appropriate external dna control (edc) is challenging. ...
در این پایان نامه کمپلکس های مس جدیدی از واکنش بنزهیدرازید و استیل استون با برخی نمک های مس(ii) تهیه شد. کلاستر مس(ii) چهارهسته ای (کمپلکس 1) با فرمول [cu4cl6o(c5h8n2)4] از واکنش استیل استون و بنزهیدرازید با نسبت مولی یک به یک و مس کلرید(ii) در متانول سنتز شد و کمپلکس 2، [cu(c19h16n4o3)] از واکنش لیگاند bzpyzn با نمک های مس (ii) در متانول با نسبت های برابر بدست آمد و تبدیل –ch= در مرکز استیل اس...
RecQ DNA helicases act in conjunction with heterologous partner proteins to catalyze DNA metabolic activities, including recombination initiation and stalled replication fork processing. For the prototypical Escherichia coli RecQ protein, direct interaction with single-stranded DNA-binding protein (SSB) stimulates its DNA unwinding activity. Complex formation between RecQ and SSB is mediated by...
Outbreaks of Marek's disease (MD) in vaccinated flocks still occur sporadically and lead to economic losses. Unfortunately, adequate methods to predict MD outbreaks are lacking. In the present study, we have evaluated whether high load of challenge MD virus (MDV) DNA in peripheral blood could aid in the early diagnosis of MD and in monitoring efficacy of vaccines against MD. One experiment was ...
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