Vitrifying oocytes is a potentially valuable means of preserving the female germ line, but significantly compromises oocyte developmental competence. This study examined the hypothesis that the cumulus complex protects the oocyte during vitrification. Vitrified-warmed immature cumulus oocyte complexes (COCs) were labelled with a plasma membrane impermeant DNA marker (ethidium homodimer-1) to ex...

The cryopreservation of liver slices permits optimal use of large livers and provides for the availability of the slices at any desired time. Moreover, it allows one to study liver slices from various species in the same experiment. We studied the effect of cryopreservation on the viability and metabolic activity of liver slices from rat, monkey, dog, hamster, minipig, rabbit and human. Precisi...

Cryopreservation of pancreatic islets enables their long-term storage and subsequent transplantation; however, post-cryopreservation, viability, functions are reduced to a significant extent. Islet is composed five cells (? cell, ? ? ? PP cell), blood vessels that carry the nutrition. Freezing technology organization has not developed good method. This paper studied using fructan which been fou...

Clinical outcomes of ICSI when using only five or fewer testicular spermatozoa after cryopreservation have been unsuccessful and are considered to be inferior those fresh from Micro-TESE. A possible cause these poor results has the lack a successful freezing technique. In cases, repeated Micro-TESE simultaneous oocyte pick up available treatment. We developed an innovative ultra-rapid vitrifica...

Sperm cryopreservation is a valuable tool for the genetic management of ex situ populations. This study was conducted to assess: (1) semen characteristics of wild-born cheetahs; and (2) the impact of three types of glycerol influence (duration of exposure, temperature, and method of addition) on sperm cryosensitivity. To evaluate the impact of duration of glycerol exposure, spermatozoa were inc...

Human bone marrow-derived mesenchymal stem cells (hBMSCs) are known for their potential to undergo mesodermal differentiation into many cell types, including osteocytes, adipocytes, and chondrocytes. Therefore, hBMSCs can be used for a variety of regenerative medicine therapies, in fact, hBMC-derived osteocytes have already been used in bone reconstruction. This study discusses the viability an...

BACKGROUND Cell transplantation for regenerative medicine has become an appealing therapeutic method; however, stem and progenitor cells are not always freshly available. Cryopreservation offers a way to freeze cells as they are generated, for storage and transport until required for therapy. This study was performed to assess the feasibility of cryopreserving peripheral blood mononuclear cells...

Abstract Study question Whether the fertility preservation can benefit male patients with cancer. Summary answer Sperm cryopreservation is an effective method and may What known already only way to efficiently preserve fertility. It important procedure in assisted reproductive technologies (ART). Recently, due remarkable advances cancer treatment, increasing number of studies have reported outc...

BACKGROUND Vitrification is now a commonly applied technique for cryopreservation in assisted reproductive technology (ART) replacing, in many cases, conventional slow cooling methodology. This review examines evidence relevant to comparison of the two approaches applied to human oocytes and embryos at different developmental stages. METHODS Critical review of the published literature using P...

Maintaining and restore the full developmental competence of either mature or immature oocytes after cryopreservation processes to preserve women fertility is a goal in assisted reproductive technologies (ART). This goal arises from the need of some patients to preserve their oocytes before arriving to a reproductively mature age, such as patients who need to undergo chemotherapy. Although in v...