نتایج جستجو برای: cryo
تعداد نتایج: 7054 فیلتر نتایج به سال:
چکیده ندارد.
In this work, we introduce persistent homology for the analysis of cryo-electron microscopy (cryo-EM) density maps. We identify the topological fingerprint or topological signature of noise, which is widespread in cryo-EM data. For low signal-to-noise ratio (SNR) volumetric data, intrinsic topological features of biomolecular structures are indistinguishable from noise. To remove noise, we empl...
Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. We demonstrate the utility of this approach to drive pa...
Independent cryo electron microscopy (cryo-EM) studies of the closely related protein disaggregases ClpB and Hsp104 have resulted in two different models of subunit arrangement in the active hexamer. We compare the EM maps and resulting atomic structure fits, discuss their differences, and relate them to published experimental information in an attempt to discriminate between models. In additio...
Since the advent of cryo-electron microscopy (cryo-EM), the close-to-life and fine structure preservation of biological samples has become a realized procurement, surpassing the fixation quality and avoiding the numerous artifacts inherent to conventional chemical preservation methods. Cryo-EM techniques such as high-pressure freezing followed by freezesubstitution (HPF/FS) have facilitated the...
We developed a method, named GraFix, that considerably improves sample quality for structure determination by singleparticle electron cryomicroscopy (cryo-EM). GraFix uses a glycerol gradient centrifugation step in which the complexes are centrifuged into an increasing concentration of a chemical fixation reagent to prevent aggregation and to stabilize individual macromolecules. The method can ...
BACKGROUND In addition to minimally invasive destruction of tumors, cryo-ablation of tumors to some extent modulated anti-tumor immunity. Cryo-ablated tumors in glioma mice models induced anti-tumor cellular immunologic response which increases the percentage of CD3(+) and CD4(+)T cells in blood as well as natural killer cells. As a crucial role in triggering anti-tumor immunity, dendritic cell...
In this paper, we provide assembly instructions for an easy-to-build experimental setup in order to gain practical experience with tomography. As such, paper can be seen as a complementary work excellent undergraduate-level mathematical textbooks concerned the basic principles of Since uses light tomographic imaging, objects investigated need transparent, such origami figures. Should reader wan...
Cryo-Electron Microscopy (Cryo-EM) images are characterized by the low signal-to-noise ratio, contrast, serious background noise, more impurities, less data, difficult data labeling, simpler image semantics, and relatively fixed structure, while U-Net obtains resolution when downsampling rate information to complete object category recognition, high-resolution during upsampling precise segmenta...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید