Cronobacter sakazakii is an opportunistic foodborne pathogen and the virulence differences were previously documented. However, information about membranous proteins involved in virulence differences was not available. In this study, virulent characterization such as biofilm formation and flagella motility between virulent C. sakazakii isolate G362 and attenuated L3101 were determined. Then, tw...

Lactobacillus acidophilus n.v. Er2 317/402 strain Narine is known as a health beneficial functional probiotic culture and supplementary source of nutrition for newborns. In this study, in vitro antimicrobial activities of Narine-lyophilized (Narine-L), Narine-heat treated (Narine-HT), and Narine crude cell-free extract (Narine-CCFE) were evaluated against pathogen Cronobacter sakazakii (C. saka...

Bacteria respond to elevated osmolality by the accumulation of a range of low molecular weight molecules, known as compatible solutes (owing to their compatibility with the cells' normal physiology at high internal concentrations). The neonatal pathogen Cronobacter sakazakii is uniquely osmotolerant, surviving in powdered infant formula (PIF) which typically has a water activity (aw) of 0.2 - i...

Lactobacillus bulgaricus prevents intestinal epithelial cell injury caused by Cronobacter 1 (Enterobacter) sakazakii-induced nitric oxide both in vitro and in the newborn rat model of 2 necrotizing enterocolitis. 3 Catherine J. Hunter 1 , Monica Williams 1 , Mikael Petrosyan 1 , Yigit Guner 1 , Rahul Mittal 2 , Dennis 4 Mock 3 , Jeffrey S. Upperman 1, 4 , Henri R. Ford 1, 4 and Nemani V. Prasad...

The presence of Cronobacter sakazakii must be controlled in infant powder plants, because it may cause infectious disease infants, with high mortality. Testing for C. powdered formula should performed before delivery, and requires rapid specific detection methods. In this study, we established a surface-enhanced Raman scattering (SERS) immunochromatographic test strip the quantitative determina...

Cronobacter sakazakii is an opportunistic pathogen that causes severe infections in neonates and infants through contaminated powdered infant formula (PIF). Therefore, the aim of this study was a large-scale study on determine the prevalence, molecular characterization and antibiotic susceptibility of C. sakazakii isolates from PIF purchased from Chinese retail markets. Two thousand and twenty ...

Cronobacteris associated with infant infections and the consumption of reconstituted infant formula. Here we sequenced and closed six genomes ofC. condimenti(T),C. muytjensii(T),C. universalis(T),C. malonaticus(T),C. dublinensis(T), andC. sakazakiithat can be used as reference genomes in single nucleotide polymorphism (SNP)-based next-generation sequencing (NGS) analysis for source tracking inv...