removal of thiosulfate (s2o32-, 200 mg/l) from simulated wastewater samples was investigated under varying co-pollutants and ph conditions employing the titanium dioxide (tio2) mediated photocatalysis. for thiosulfate only studies higher substrate removal was noted at lower ph with a specific trend of ph 4 > ph 7 > ph 10. furthermore, near complete thiosulfate removal was also achieved at ph 4....

Molecular Modeling of the Metabolism of Acetaminophen and Acetaminophen-like Molecules by Laura Rebecca Hofto Paracetamol, or acetaminophen, is a common analgesic and fever-reducer. There are three major metabolic pathways for acetaminophen in the liver. The final products of each pathway are nontoxic and are excreted by the kidneys. Cresol and similar molecules are competitors with acetaminoph...

Olsenella scatoligenes SK9K4(T) is a strictly anaerobic bacterium isolated from pig feces that produces the malodorous compounds 3-methylindole (skatole) and 4-methylphenol (p-cresol). Here, we report the 2.47 Mbp draft genome sequence of SK9K4(T), exploring pathways for the synthesis of skatole and p-cresol from the amino acids tryptophan and tyrosine, respectively.

Anoxic cell extracts of a denitrifying bacterial isolate (PC-07) were shown to oxidize p-cresol to p-hydroxybenzoate. Oxidation of the substrate was independent of molecular oxygen and required nitrate as the natural terminal electron acceptor. Two enzyme activities were implicated in the pathway utilized by PC-07. A p-cresol methylhydroxylase mediated the oxidation of p-cresol to p-hydroxybenz...

Endothelial dysfunction and impaired endothelial regenerative capacity are key contributors to the high incidence of cardiovascular disease in patients with chronic kidney disease (CKD). Uremic toxins are associated with this pathogenesis. Previous studies have revealed that a uremic toxin, para‑cresol (p‑cresol), exerts an antiproliferation effect on human endothelial progenitor cells (EPCs), ...

for AICHE 2004 [15C12] Advances in Biocatalysis and Protein Engineering Tailoring Toluene para-Monooxygenase of Ralstonia pickettii PKO1 for Regiospecific Oxidation of Aromatics Using Active Site Engineering A. Fishman, Y. Tao, W. E. Bentley, and T. K. Wood University of Connecticut, Storrs, CT University of Maryland, College Park, MD Oxygenases are promising biocatalysts for performing selecti...

Toluene-o-xylene monooxygenase (ToMO) from Pseudomonas stutzeri OX1 oxidizes toluene to 3- and 4-methylcatechol and oxidizes benzene to form phenol; in this study ToMO was found to also form catechol and 1,2,3-trihydroxybenzene (1,2,3-THB) from phenol. To synthesize novel dihydroxy and trihydroxy derivatives of benzene and toluene, DNA shuffling of the alpha-hydroxylase fragment of ToMO (TouA) ...

Although there are more than 200 odor-causing volatile organic compounds (VOCs), phenol and p-cresol are two prominent odor-causing VOCs found downwind from concentrated animal feeding operations (CAFOs). The VOC emissions from cattle and dairy production are difficult to quantify accurately because of their low concentrations, spatial variability, and limitations of available instruments. To q...

Oxygenases are promising biocatalysts for performing selective hydroxylations not accessible by chemical methods. Whereas toluene 4-monooxygenase (T4MO) of Pseudomonas mendocina KR1 hydroxylates monosubstituted benzenes at the para position and toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 hydroxylates at the ortho position, toluene 3-monooxygenase (T3MO) of Ralstonia pickettii P...

Skin reactions at the infusion site are a common side effect of continuous subcutaneous insulin infusion therapy. We hypothesized that local skin complications are caused by components of commercial insulin formulations that contain phenol or m-cresol as excipients. The toxic potential of insulin solutions and the mechanisms leading to skin reactions were explored in cultured cells. The toxicit...