The effect of growth regulators and culture conditions on the morphogenetic response of cotyledonary leaf discs was studied in popular cucumber variety (Cucumis sativus cv. Sheetal). Organogenesis was induced directly without any intervening callus phase on Murashige and Skoog medium supplemented with different concentrations of benzyladenine and indole propionic acid. Best results (93%) were o...

Greenhouse and field experiments showed that conidia of the fungal pathogen, Phoma commelinicola, exhibited bioherbicidal activity against spreading dayflower (Commelina diffusa) seedlings when applied at concentrations of 10 to 10 conidia·mL. Greenhouse tests determined an optimal temperature for conidial germination of 25 °C–30 °C, and that sporulation occurred on several solid growth media. ...

Callus induction, somatic embryogenesis and plant regeneration were initiated in selected five species of Turkish crocus using three diffrent explants (leaf, stem and corm) cultured on four different media (MS, GB5, LS and CHE). The highest frequencies of callus induction (100%) and shoot regeneration (70%, with 7.2 shoots/callus) were found in the crocus species Crocus oliveri ssp. Oliveri, us...

Young seedlings of Lavatera cretica L. exhibit positive phototropism. The hypocotyl perceives unilateral illumination with blue light and curves towards the light source by unequal growth. In addition, the cotyledonary laminas perceive the vectorial component of unilateral illumination with blue light and reorient normal to the beam by creating a turgor differential in their pulvini. Excision o...

A protocol for direct organogenesis in Bixa orellana (pink flowers variety) has been developed with significant organogenic response from rooted hypocotyls, hypocotyl segments, and cotyledonary leaf explants on Murashige and Skoog (MS) medium supplemented with 2.0 mg L thidiazuron and 0.25% coconut water or 7.0 mg L N-Benzyladenine and 0.1 mg L a-naphthalene acetic acid. Thidiazuron in combinat...

The present work aimed at regenerating plants of Eucalyptus camaldulensis from the cotyledonary explants and describing the anatomy of the tissues during callogenesis and organogenesis processes, in order to determine the origin of the buds. The cotyledonary leaves of E. camaldulensis were cultured in Murashige and Skoog (MS), WPM and JADS media supplemented with 2.7 μM NAA and 4.44 μM BAP. The...

This experiment was performed to evaluate the effects of different types and concentrations of carbohydrate source on callus fresh weight, number of somatic embryos per embryonic explant and percentage of globular embryos developing into cotyledonary embryos from the leaf explants of three strawberry cultivars (Kurdistan, Paros and Camarosa). MS medium containing 1.0 mg/l 2,4-dichlorophenoxyace...

The results of studies on in vitro regeneration of chickpea (Cicer arietinum L.) have shown that indirect regeneration via callus was not successful. The introduction of an efficient and repeatable regeneration protocol is necessary for using transgenic technologies for the improvement of chickpea. The present study was undertaken to investigate the possibility of indirect regeneration in vario...

low phosphorous (p) availability in soils limits production of soybean [glycine max (l.) merr.] around the world. this study was conducted to determine whether exogenous expression of the rice (oryza sativa l.) phosphates transporter gene ospt2 would increase inorganic phosphates (pi) acquisition and improve yield in transgenic soybean. cotyledonary-node explants of the soybean were inoculated ...