نتایج جستجو برای: core promoter

تعداد نتایج: 301283  

Journal: :Journal of virology 2000
S Gilbert L Galarneau A Lamontagne S Roy L Bélanger

Orphan nuclear receptor fetoprotein transcription factor (FTF) was previously identified as a specific regulator of the alpha(1)-fetoprotein gene during early liver development and in response to hormonal signals (L. Galarneau, J.-F. Paré, D. Allard, D. Hamel, L. Lévesque, J. D. Tugwood, S. Green, and L. Bélanger, Mol. Cell. Biol. 16:3853-3865, 1996). Here we report a functional analysis of FTF...

Journal: :Molecular cell 2016
Natalia Petrenko Yi Jin Koon Ho Wong Kevin Struhl

Mediator is a transcriptional co-activator recruited to enhancers by DNA-binding activators, and it also interacts with RNA polymerase (Pol) II as part of the preinitiation complex (PIC). We demonstrate that a single Mediator complex associates with the enhancer and core promoter in vivo, indicating that it can physically bridge these transcriptional elements. However, the Mediator kinase modul...

2007
Akiko Kobayashi Youko Watanabe Koji Akasaka Tetsuro Kokubo

There are some functional compatibilities between upstream and core promoter sequences for transcriptional activation in yeast, Drosophila and mammalian cells. Here we examined whether similar compatibilities exist in sea urchin embryos, and if so, whether they are dynamically regulated during early development. Two reporter plasmids, each containing a test promoter conjugated to either CFP or ...

2015
Wang Yao Yinxia Li Bojiang Li Hua Luo Hongtao Xu Zengxiang Pan Zhuang Xie Qifa Li Stefan Schlatt

Non-primate mammals have two deleted azoospermia (DAZ) family genes, DAZL and Boule; genes in this family encode RNA-binding proteins essential for male fertility in diverse animals. Testicular DAZL transcription is regulated by epigenetic factors such as DNA methylation. However, nothing is known about the epigenetic regulation of Boule. Here, we explored the role of DNA methylation in the reg...

Journal: :The Journal of biological chemistry 2001
S K Ogden K C Lee K Wernke-Dollries S A Stratton B Aronow M C Barton

Many of the functions ascribed to p53 tumor suppressor protein are mediated through transcription regulation. We have shown that p53 represses hepatic-specific alpha-fetoprotein (AFP) gene expression by direct interaction with a composite HNF-3/p53 DNA binding element. Using solid-phase, chromatin-assembled AFP DNA templates and analysis of chromatin structure and transcription in vitro, we fin...

Journal: :Molecular and cellular biology 2000
Y Tsukihashi T Miyake M Kawaichi T Kokubo

The general transcription factor TFIID, which is composed of TATA-binding protein (TBP) and an array of TBP-associated factors (TAFs), has been shown to play a crucial role in recognition of the core promoters of eukaryotic genes. We isolated Saccharomyces cerevisiae yeast TAF145 (yTAF145) temperature-sensitive mutants in which transcription of a specific subset of genes was impaired at restric...

Journal: :Genes & development 2014
Adi Kedmi Yonathan Zehavi Yair Glick Yaron Orenstein Diana Ideses Chaim Wachtel Tirza Doniger Hiba Waldman Ben-Asher Nemone Muster James Thompson Scott Anderson Dorit Avrahami John R Yates Ron Shamir Doron Gerber Tamar Juven-Gershon

Transcription of protein-coding genes is highly dependent on the RNA polymerase II core promoter. Core promoters, generally defined as the regions that direct transcription initiation, consist of functional core promoter motifs (such as the TATA-box, initiator [Inr], and downstream core promoter element [DPE]) that confer specific properties to the core promoter. The known basal transcription f...

Journal: :Nucleic acids research 1990
S. Firek C. Read D. R. Smith Tom Moss

The core region of the Xenopus laevis pre-ribosomal RNA promoter was subjected to point mutation analysis. A total of 27 point mutants within a 78 base pair region from -64 to +14, (relative to the start of transcription at +1), were assayed by oocyte microinjection. The results locate the 3' boundary of the core promoter at +4 and the 5' boundary at between -33 and -39 and suggest that this re...

Journal: :cell journal 0

objective: mir-302-367 is a cluster of polycistronic micrornas that are exclusively expressed in embryonic stem (es) cells. the mir-302-367 promoter is functional during embryonic development but is turned off in later stages. motivated by the cancer stem cell hypothesis, we explored the potential expression of mir-302 in brain tumor cell lines. materials and methods: in the present experimenta...

Journal: :Genes & development 2008
Jer-Yuan Hsu Tamar Juven-Gershon Michael T Marr Kevin J Wright Robert Tjian James T Kadonaga

The RNA polymerase II core promoter is a structurally and functionally diverse transcriptional module. RNAi depletion and overexpression experiments revealed a genetic circuit that controls the balance of transcription from two core promoter motifs, the TATA box and the downstream core promoter element (DPE). In this circuit, TBP activates TATA-dependent transcription and represses DPE-dependen...

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