Human brucellosis is a significant public health problem in many Mediterranean countries including Greece. The conventional serological methods, as well as blood cultures, have serious limitations, especially in chronic, relapsing, and focal forms of the disease. Four different PCR assays were applied in 4,926 buffy coat, whole-blood, and serum samples received from 200 patients admitted with b...

Case Report Mrs. Vail is group 0, Rh-negative, Duffy-negative; her husband, group 0, Rh-negative, Duffy-positive. Mr. Vall is 0; MsNs; P+; cdE.cde; kk; Fy(a+); Le(a-). Mrs. Vall is 0; NsNs; P+; cde.cde; Kk; Fy(a-); Le(a-); Jk(a+). Baby is 0; NsNs; P+; cde.cde; kk; Fy(a+); Le(a-). Mrs. Vail's pregnancy record is as follows: (1) In 1949, normal. The mother was transfused immediately following thi...

More than a decade ago it was recognized that ACTH and cortisone ameliorate some hemolytic anemias in man (1-6). Nevertheless, the mechanisms responsible for their therapeutic effect have not been elucidated. I t is generally believed that cortisone and its analogues benefit only those hemolytic anemias in which the red cells can be agglutinated by Coombs (antiglobulin) serum. Indeed, of the ma...

Severe hemolytic anemia in patients with disseminated tuberculosis is exceedingly rare. We report an episode of Coombs'-positive hemolytic anemia in a previously healthy young man with miliary tuberculosis, resulting in a hemoglobin level of 5 g/dL and an undetectable haptoglobin level. The patient responded well to treatment with antituberculosis drugs, and the results of the direct Coombs' te...

Background. Brucellosis is one of the most common diseases of humans and animals and its clinical manifestations differ from asymptomatic infection to chronic illness associated with recurrence of symptoms. This study aimed to evaluate the prevalence of brucellosis in asymptomatic children 7 to 12 years old in Kahak, Iran. Methods. In this study, 186 children 7 to 12 years old were evaluated. D...

Purpose. Acute scrotal pain as the first symptom of brucellosis is rarely observed. We aimed to evaluate the data of male patients with brucellosis and epididymoorchitis as the initial diagnosis. Material and Methods. The data of seven patients presented with testicular pain, hyperemia, swelling, and increased fever were reviewed. Concomitant focal diseases as well as clinical, laboratory, and ...

The specific agglutination by incomplete anti D antibodies of a saline suspension of red cells, after preliminary incubation with a solution of crystalline trypsin, has been previously reported (Morton and Pickles, 1947). During the past two years this method has been standardized and used as a test for the detection of incomplete antibodies. The trypsin-treated cells have been found to give re...

During the last ten years the extraction of specific antibodies (ab) from the yolk of eggs of immunised chickens is more and more accepted as an useful alternative to the immunisation of mammals. The subject of this work is the immunisation of chickens with human IgG and the extraction of specific anti human IgG ab from egg yolk in order to obtain monospecific Coombs reagent. 12 Leghorn hens (2...