A PCR based reverse transcriptase (RT) assay was developed that has 10(4)-fold higher sensitivity than conventional nucleotide incorporation assays and allows discrimination between false positive results generated by cellular polymerases and positives resulting from authentic RT activity. Recently, several reverse transcriptase (RT) assays have been developed where a reverse transcriptase reac...

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 10(1) 50% egg infection dose (EID(50)) per 50 microl of titrated viruses and no cross-reaction of IBV RT-LAMP was found when tested with other viruses including Newcas...

Patients with residual or recurrent Cushing's disease receive external beam radiotherapy (RT) with the aim of achieving long-term tumour control and normalization of elevated hormone levels. Treatment is given either as conventional radiotherapy using conformal techniques or as stereotactic radiotherapy, which is either used as fractionated treatment (SCRT) or as single fraction radiosurgery (S...

This study was aimed at analyzing the yield, grain quality and weed infestation of spring triticale sown in 3 tillage systems: a) conventional tillage (CT)-shallow ploughing and harrowing after harvest of the previous crop, ploughing in the autumn; b) reduced tillage (RT)-only cultivator after harvest of the previous crop, and c) no-tillage (NT)-only glyphosate (360 g L-1) after harvest of ...

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription- PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swi...

This study was initiated in 1991 at the Kansas State University Southwest Research-Extension Center near Tribune, KS. The purpose of to identify effects tillage intensity on precipitation capture, soil water storage, and grain yield a wheat-sorghum-fallow rotation. Grain yields wheat sorghum increased with decreased wheat-sorghumfallow (WSF) In 2022, available planting greater for no-tillage (N...