BACKGROUND The sensitivity and specificity of 18S rRNA polymerase chain reaction (PCR) in the detection of fungal aetiology of microbial keratitis was determined in thirty patients with clinical diagnosis of microbial keratitis. METHODS Corneal scrapings from patients were used for Gram stain, culture and PCR analysis. PCR was performed with primer pairs targeted to the 18S rRNA gene. The res...

the presence of helicobacter pullorum in intestinal tract of broiler chickens may be a potential risk for human health. in this study, a total of 100 caecal samples of broilers carcasses from 20 flocks at a poultry abattoir in mashhad suburb were tested for the presence of h. pullorum using modified conventional culture method by combination of culturing on brucella sheep blood agar and a filte...

To identify the bacterial diversity (microbiota) in expectorated sputum, a pyrosequencing method that investigates complex microbial communities of expectorated sputum was done in 19 stable chronic obstructive pulmonary disease patients (mean (SD) FEV1: 47 (18%) of predicted value). Using conventional culture, 3 phyla and 20 bacterial genera were identified, whereas the pyrosequencing approach ...

conclusions it can be concluded that a. otitidis was the major causative agent of mee in children with ome. therefore clinicians should be aware that bacterial infection plays an important role in the progression of acute otitis media to ome in children of our region. results the bacterial cultures in 8.6% of samples were positive by conventional culture, with a. otitidis, m. catarrhalis and s....

Two 48-h Vero cell systems were compared with viral culture in human fibroblastic cells for isolation and identification of herpes simplex virus from clinical specimens. Both 48-h systems had 79% sensitivity and greater than 99% specificity as compared with the conventional tissue culture method.

Resistance of Mycobacterium tuberculosis (MTB) to antituberculous drugs has been threatening the public-health grossly. Recently, because of high-level of resistance to rifampicin in MTB, treatment is becoming impossible. In this study, 127 rifampicin resistant and 33 rifampicin susceptible strains were randomly chosen from tuberculosis culture-positive isolates, isolated in the laboratory of t...

The purpose of this study was to examine the potential application of Sorbitol, Sucrose and Gelatin in combination with glycerol in order to Theileria annulata infected cell line cryo-preservation. The efficiency of the freezing methods was compared by assessing the viability and plating efficiency of cells after resuscitation, dilution and equilibration in cell culture medium. As a result, the...

The Addi-Chek (filtration; Millipore Corp., Bedford, Mass.) and BACTEC (radiometric detection of growth in culture media; Johnston Laboratories, Inc., Towson, Md.) systems were compared with the 10-ml culture (centrifugation) method for the recovery of microorganisms from peritoneal dialysate collected from patients with clinical evidence of peritonitis and containing greater than or equal to 2...