Early erythroid precursor cells and myaloid progenitor cells (CFU-GM) from four patients with 5q syndrome ware cultured in order to study the in vitro growth patterns and to determine the clonal origin of this hamatologic disorder. Cultures of CFU-GM exhibited normal colony growth. while erythroid progenitor cells demonstrated a marked decrease or absence of colony growth. Chromosomal studas in...

The response of human myeloid leukemia cells to various sources of colony-stimulating activity (CSA) and media conditioned by phytohemagglutinin-stimulated mononuclear cells (PHA-LCM) was investigated in liquid and colony culture. PHA-LCM, placenta-conditioned medium, GCT cell line-conditioned medium, leukocyte-conditioned medium, and partially purified CSA for human and murine cells were teste...

Nerve growth factor (NGF) is a neurotropic polypeptide necessary for the survival and growth of some central neurons, as well as sensory afferent and sympathetic neurons. Much is now known of the structural and functional characteristics of NGF, whose gene has recently been cloned. Since it is synthesized in largest amounts by the male mouse submandibular gland, its role exclusively in nerve gr...

nine organic substrates viz., rice grains, sorghum grains, wheat grains, millet grains, wheat straw, rice husk, cow dung, sawdust, and poultry manure were used for mass multiplication of trichoderma harzianum. of these,sorghum grains followed by millet grains were the best substrates. the poultry manure appeared to be the most unsuitable substrate, whereas rice grains, wheat grains, wheat straw...

Raf-1 is a 74-kD serine/threonine kinase located in the cell cytoplasm that is activated by phosphorylation in cells stimulated with a variety of mitogens and growth factors, including hematopoietic growth factors. Using c-raf antisense oligonucleotides to block Raf-1 expression, we have established that Raf-1 is required for hematopoietic growth factor-induced proliferation of murine cell line...

Tumor-promoting phorbol esters stimulated mouse bone marrow cells to form myeloid colonies in agar cultures without added colony-stimulating factors. The colony-stimulating ability of various phorbol esters correlated well with their ability to promote skin tumors in vivo. These results suggest that phorbol esters mimic the action of specific colony-stimulating factors that regulate growth.

background: yeast infections are increasing cause of morbidity and mortality in immunocompromised patients. in order to perform a dna-based diagnostic test, availability of a rapid and easy-to-perform dna extraction protocol is essential. in the present study we evaluated colony-pcr as the easiest way to amplification of target dna. methods: instead of using templates of purified genomic dna, w...

We have investigated the periodic colony growth of bacterial species Bacillus subtilis. A colony grows cyclically with the interface repeating an advance (migration phase) and a rest (consolidation phase) alternately on a surface of semi-solid agar plate under appropriate environmental conditions, resulting in a concentric ring-like colony. It was found from macroscopic observations that the ch...

Sunn pest is one of the most important cereal pests that affect both grain yield and quality of cerealin Iran. One potential control strategy is to use entomopathogenic fungi such as Beauveriabassiana Vuillemin as mycoinsecticide to control this pest. Naturally infested Sunn pests inKermanshah province were collected and brought to laboratory. 10 isolates were selected forfurther analysis in th...

Some of the phenotypic characteristics of a slow-growing, nongerminating variant of a commonly studied strain of Candida albicans are described. The variant arose as a chance isolate. The rate of occurrence was about 0 X 1% and the reversion rate was about 1 per 10(6) cells. The colony size was typically smaller than that of the parent and the yeast cells tended not to separate from one another...