this study investigated biodegradation of dibenzothiophene (dbt) in marsh soil spiked bywhite-rot fungus phanerochaete chrysosporium. soil samples were spiked with 100 ppm dbtand incubated at 30°c in a dark chamber for 30 days. samples were evaluated for ph, mnperoxidaseactivity, sulfate ion concentration and growth during the tests. results showedmaximum levels of ph, mn-peroxidase and sulfate...

OBJECTIVES CD34+ cells and colony forming unit-granulocyte and macrophage (CFU-GM) from human bone marrow were used to investigate the role of Fas/FasL system in the regulation of myelopoiesis. METHODS Fas and FasL expression in CD34+ cells and in day 14 CFU-GM were measured by RT-PCR and immunofluorescence respectively. The functional assays for the CFU-GM were measured by a standard colony ...

We read with interest the article “Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principles” by Yoder et al.1 The authors compared 2 types of colony assays that have been shown to identify endothelial progenitor cells (EPCs)2,3 and studied the function of the cells derived from these assay systems. They found that a commercially available kit...

The assessment of nonviable haematopoietic cells by Annexin V staining method in flow cytometry has recently been published by Duggleby et al. Resulting in a better correlation with the observed colony formation in methylcellulose assays than the standard ISHAGE protocol, it presents a promising method to predict cord blood potency. Herein, we applied this method for examining the parameters du...

Tritium RBE/Tritiated Water/CFUs/CFUc The acute effects on hematopoietic tissue in mice of a single administration of tritiated water were compared with the effects of exposure to 6OCo gamma radiation. The activity of tritium in the bone marrow was measured directly using a sample oxidizer and the absorbed dose was calculated from the amount of activity of tritium in it. The number of nucleated...

We assessed two procedures for the initial processing of urine specimens: (i) manual screening of urine samples by inoculation into tryptic soy broth and incubation for 3 to 6 h, and (ii) examination of agar plates inoculated with urine and incubated for 4 to 6 h. Of the urine samples with greater than 10(5) colony-forming units per ml, 73.6 and 90.1% were detected with the broth cultures after...

Standard methods for quantification of airborne bacteria are based on cultivation and counting of grown colonies. From complex natural environments it is known that only a small fraction of the total number of cells can be cultivated on routinely used agar-media. Direct microscopic cell counting after DNA-staining usually generates higher concentrations of one to two magnitudes. The objective o...

OBJECTIVES To determine the quantity and quality of bacterial and fungi on money coins and to identify those that could pose a public health risk. DESIGN Random sampling of coins from subjects within predetermined categories. SETTING Westlands division of Nairobi Metropolitan province. SUBJECTS Twenty-shilling coin samples were collected from matatu (a common commuter vehicle in Kenya) ta...