نتایج جستجو برای: colony formation assay
تعداد نتایج: 776708 فیلتر نتایج به سال:
MicroRNAs regulate self renewal and differentiation of cancer stem cells. There, we sought to identify the expression of miR-181b in glioma stem cells and investigate the biological effect of miR-181b on glioma stem cells in this study. MiR-181b expression was measured by real-time PCR in glioma stem cells isolated from U87 cells by FACS sorting. After miR-181b was overexpressed in U87 glioma s...
Activin A, a member of the transforming growth factor (TGF)-beta superfamily, is involved in the regulation of erythroid differentiation. Previous studies have shown that activin A inhibited the colony-forming activity of mouse Friend erythroleukemia cells, however, the mechanism remains unknown. First, we show herein that activin A induced the expression and activated the promoters of alpha-gl...
CIonal assays for CFU-S, CFU-C, BFU-E, and CFU-E were employed to evaluate the effect of ouabain on the proliferation of mouse hematopoietic progenitor cells. Preincubation of bone marrow cells with ouabain at concentrations of 101_1012 M suppressed the proliferation of CFU-S as measured by the spleen colony assay. At lO9Mouabain, spleen colony formation was inhibited by more than 95%. When inc...
Serum stimulates both Ca2+ mobilization and colony growth of many small cell lung cancer (SCLC) cell lines, but the factors involved remain unknown. We demonstrate that 1-oleoyl-lysophosphatidic acid (LPA), like serum, induced a dose-dependent increase in intracellular Ca2+ in the H-510, H-345, and H-69 SCLC cell lines with half maximal concentrations of 18 nM, 22 nM, and 20 nM, respectively. T...
Radiotherapy plays an important role in the treatment of hepatocellular carcinoma (HCC). Cyclin G1 is a novel member cyclin family, and it abnormally expressed HCC. In this study we investigated radiotherapy HCC cells. The expression was silenced by transfection G1-siRNA into HepG2 cells Huh7 cells, mRNA protein measured qRT-PCR Western blot analysis. proliferation analyzed using MTT assay, rad...
The mitotic spindle checkpoint (SAC) genes have been considered targets of anticancer therapies. Here, we sought to identify the attractive mitotic spindle checkpoint genes appropriate for human hepatocellular carcinoma (HCC) therapies. Through expression profile analysis of 137 selected mitotic spindle checkpoint genes in the publicly available microarray datasets, we showed that 13 genes were...
Aberrant activation of beta-catenin/TCF (T-cell factor) signaling is frequently observed in the pancreatic cancer. However, the regulation of nuclear beta-catenin/TCF transcription machinery remains largely unknown. In this study, TFCP2 (transcriptional factor CP2) expression in pancreatic cancer was detected by qPCR, immunohistochemistry and western blot. Western blot, colony formation assay, ...
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