The detyrosination/retyrosination cycle is the most common post-translational modification of α-tubulin. Removal conserved C-terminal tyrosine α-tubulin by a still elusive tubulin carboxypeptidase, and religation this ligase (TTL), are probably to all eukaryotes. Interestingly, for plants, only candidates qualifying as potential TTL homologs ligase-like 12 proteins. To get insight into biologic...

Segments of coleoptiles of 3-day-old wheat (Triticum x Aestivum L. cv. Kharkov M.C. 22) grown at 24 C were strung on a glass rod and the kinetics of their elongation in 0.01 m K-phosphate buffer was examined photometrically. Measured rates of elongation in response to treatments were corrected by subtraction of endogenous rates. The customary practice of testing the effects of growth regulators...

Rhythmic fluctuation in the basipetal movement of auxin occurs in corn (Zea mays) coleoptiles oriented either in the vertical or in the horizontal position. This periodicity of transport rate varies from region to region in a horizontal coleoptile. Between an upper and lower half coleoptile (with respect to gravity), the comparable regions in the coleoptile do not exhibit similar periods. The v...

We studied the temporal and spatial pattern of lipid transfer protein (LTP) gene expression, as well as the localization of this protein, in maize. Using an LTP gene, we observed an accumulation of LTP mRNA in embryos and endosperms during seed maturation. LTP gene expression was also investigated in young seedlings. After germination, the level of LTP mRNA in the coleoptile increased, with a m...

Uridine diphosphate glucose pyrophosphorylase was isolated separately from Avena sativa leaves, roots, and etiolated coleoptiles and purified by ammonium sulfate fractionation, DEAE-Sephadex chromatography and polyacrylamide gel electrophoresis. There was no difference in the enzyme from the different tissue types with respect to properties exhibited during the purification procedure. A small p...

Metasequoia glyptostroboides Hu et W.C. Cheng, known as a living fossil species, is planted in parks, gardens, and streets many temperate regions worldwide. Adequate branch pruning necessary to grow the plants these locations, generates large amount of waste. In this study, allelopathic activity pruned-branch waste was investigated search for beneficial applications The leaves M. obtained from ...

During anoxia, cytoplasmic pH regulation is crucial. Mechanisms of pH regulation were studied in the coleoptile of rice exposed to anoxia and pH 3.5, resulting in H(+) influx. Germinating rice seedlings survived a combination of anoxia and exposure to pH 3.5 for at least 4 d, although development was retarded and net K(+) efflux was continuous. Further experiments used excised coleoptile tips (...