نتایج جستجو برای: codon preference optimization

تعداد نتایج: 401777  

2012
Dharmendra Kumar Chaudhary Indra Mani Vijai Singh

Human papillomavirus (HPV) is an etiologic agent of the uterine cervix cancer and several other neoplasias in women globally. E6 protein of HPV type 16 is highly conserved and plays the key role in an inducing cancer via suppressing activity of P. We have used different bioinformatics tools for generation of phylogenetic tree, modeling of RNA secondary structure, gene designing and codon optimi...

Journal: :J. Discrete Algorithms 2011
Anne Condon Chris Thachuk

Abstract. It is now common to add protein coding genes into cloning vectors for expression within non-native host organisms. Codon optimization supports translational efficiency of the desired protein product, by exchanging codons which are rarely found in the host organism with more frequently observed codons. Motif engineering, such as removal of restriction enzyme recognition sites or additi...

2017
Manuel Hiss Lucas Schneider Christopher Grosche Melanie A. Barth Christina Neu Aikaterini Symeonidi Kristian K. Ullrich Pierre-François Perroud Mareike Schallenberg-Rüdinger Stefan A. Rensing

The moss Physcomitrella patens is used both as an evo-devo model and biotechnological production system for metabolites and pharmaceuticals. Strong in vivo expression of genes of interest is important for production of recombinant proteins, e.g., selectable markers, fluorescent proteins, or enzymes. In this regard, the choice of the promoter sequence as well as codon usage optimization are two ...

Journal: :Computational and Structural Biotechnology Journal 2020

2015
Sukanya Jeennor Pattsarun Cheawchanlertfa Sarinya Suttiwattanakul Sarocha Panchanawaporn Chanikul Chutrakul Kobkul Laoteng

BACKGROUND The ∆(6)-desaturase gene, encoding a key enzyme in the biosynthesis of polyunsaturated fatty acids, has potential in pharmaceutical and nutraceutical applications. RESULTS The ∆(6)-desaturase gene has been isolated from a selected strain of Oomycetes, Pythium sp. BCC53698. The cloned gene (PyDes6) contained an open reading frame (ORF) of 1401 bp encoding 466 amino acid residues. Th...

2016
Eneko Villanueva Maria Martí-Solano Cristina Fillat

Codon usage adaptation of lytic viruses to their hosts is determinant for viral fitness. In this work, we analyzed the codon usage of adenoviral proteins by principal component analysis and assessed their codon adaptation to the host. We observed a general clustering of adenoviral proteins according to their function. However, there was a significant variation in the codon preference between th...

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