In order to investigate the effect of human Fallopian tube epithelial cell co-culture on fertilization and cleavage rates in tubal, male and unexplained infertility, oocytes collected from 91 patients were randomized to wells containing Fallopian tube epithelial cell monolayers or conventional culture medium, and inseminated with spermatozoa. Fertilization and cleavage were assessed at 18 and 5...

There are different views on the relationship between language and culture. Some consider them as separate entities one being a code-system and the other a system of beliefs and attitudes. Some believe in a cause and effect relationship between the two; and yet others argue for a co-evolutionary mode of interrelation. This paper will subscribe to the Hallidayan co-evolutionary view of the relat...

Objective(s): Three-dimensional biomimetic scaffolds have widespread applications in biomedical tissue engineering due to similarity of their nanofibrous architecture to native extracellular matrix. Co-culture system has stimulatory effect on chondrogenesis of adult mesenchymal stem cells. This work presents a co-culture strategy using human articular chondrons and adipose-derived stem cells (A...

Intercellular interactions are studied using different co-culture systems. Tumor conditioned medium-based systems, filter inserts and direct contact co-culture systems are often used according to research needs. In this article we present a new co-culture technique, using negatively-charged slides (NCS) as the culture surface. The technique was developed on a human-derived endothelial cell line...

Patients with repeated implantation failures or poor embryo quality may benefit from embryo culture using the co-culture technique; growth factors secreted by co-culture cells may act as survival factors. Autologous endometrial co-culture has been suggested as a safe alternative to animal cells for co-culture of human embryos. However, the technique is fairly labour intensive and its effectiven...

BACKGROUND There is a need to develop an in vitro skin models which can be used as alternative system for research and testing pharmacological products in place of laboratory animals. Therefore to study the biology and pathophysiology of pigmentation and vitiligo, reliable in vitro skin pigmentation models are required. AIM In this study, we used primary cultured melanocytes and keratinocytes...

BACKGROUND This study was conducted to evaluate fibroblast co-culture and Activin A on in vitro maturation and fertilization of mouse preantral follicles. METHODS The ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-MEM as based medium for 12 days. A total number of 456 follicles were cultured in four conditions: (i) base mediu...