The eukaryotic CMG (Cdc45, Mcm2-7, GINS) helicase consists of the Mcm2-7 hexameric ring along with five accessory factors. The Mcm2-7 heterohexamer, like other hexameric helicases, is shaped like a ring with two tiers, an N-tier ring composed of the N-terminal domains, and a C-tier of C-terminal domains; the C-tier contains the motor. In principle, either tier could translocate ahead of the oth...

Disassembly of the Cdc45-MCM-GINS (CMG) DNA helicase, which unwinds the parental DNA duplex at eukaryotic replication forks, is the key regulated step during replication termination but is poorly understood. In budding yeast, the F-box protein Dia2 drives ubiquitylation of the CMG helicase at the end of replication, leading to a disassembly pathway that requires the Cdc48 segregase. The substra...

The Cdc45-MCM-GINS (CMG) helicase unwinds DNA during the elongation step of eukaryotic genome duplication and this process depends on the MCM ATPase function. Whether CMG translocation occurs on single- or double-stranded DNA and how ATP hydrolysis drives DNA unwinding remain open questions. Here we use cryo-electron microscopy to describe two subnanometre resolution structures of the CMG helic...

The eukaryotic replisome is α multiprotein machine that contains DNA polymerases, sliding clamps, helicase, and primase along with several factors that participate in cell cycle and checkpoint control. The detailed structure of the 11-subunit CMG helicase (Cdc45/Mcm2-7/GINS) has been solved recently by cryoEM single-particle 3D reconstruction and reveals pumpjack motions that imply an unexpecte...

This paper proposes a new electronic-continuously variable transmission (E-CVT) system for power-split hybrid electric vehicles (HEVs). The key is to integrate two permanent magnet motor/generators (M/Gs) together with a coaxial magnetic gear (CMG). By designing the modulating ring of the CMG to be rotatable, this integrated machine can achieve both power splitting and mixing, and therefore, ca...

The replisome unwinds and synthesizes DNA for genome duplication. In eukaryotes, the Cdc45-MCM-GINS (CMG) helicase and the leading-strand polymerase, Pol epsilon, form a stable assembly. The mechanism for coupling DNA unwinding with synthesis is starting to be elucidated, however the architecture and dynamics of the replication fork remain only partially understood, preventing a molecular under...

Abstract Thermal injection methods are usually used for high viscosity oil. The results of previous studies showed that the combination SF and SFF had highest increase in oil recovery but still requires further study to determine optimum strategy. This work is purposed optimize development scenario a combined CSS-SF applied heavy field located Sumatera, Indonesia. factor NPV become objective fu...

BACKGROUND Clinicians have difficulty accurately assessing medication non-adherence within chronic disease care settings. Health information technology (HIT) could offer novel tools to assess medication adherence in diverse populations outside of usual health care settings. In a multilingual urban safety net population, we examined the validity of assessing adherence using automated telephone s...

ANALYSIS OF A DUAL SCISSORED-PAIR,VARIABLE-SPEED,CONTROL MOMENT GYROSCOPE DRIVEN SPHERICAL ROBOTbyRICHARD CHASEMay 2014 Advisor: Dr. Abhilash PandyaMajor: Electrical EngineeringDegree: Doctor of Philosophy The objective of this research is to compare barycenter offset based designs of sphericalrobots to control moment gyroscope (CMG) based designs in order to determi...