Confocal microscopy o ers several advantages over conventional optical microscopy with its small depth-ofeld, its reduction of out-of-focus blur, and its full three-dimensional (3D) image scanning ability. For biomedical applications, it can also acquire images of living cells, usually labeled with one or more uorescent probes. The confocal laser scanning microscope (CLSM) is an optical uoresce...

The present research analyzed the reciprocating instrumentation associated to chlorhexidine (CHX) substantivity as its correlation with E. faecalis viability in ex vivo root canals. Eighty extracted single-rooted human teeth were used, being 40 high-performance liquid chromatography (HPLC) and confocal laser scanning microscopy (CLSM). In both, decoronated cervical third was prepared. CLSM anal...

Abstract This global feasibility study assesses the potential of coarse-scale, gridded soil water estimates for probabilistic modeling hydrologically triggered landslides, using Soil Moisture Ocean Salinity (SMOS), Active Passive (SMAP), and Gravity Recovery Climate Experiment (GRACE) remote sensing data; Catchment Land Surface Model (CLSM) simulations; six data products based on assimilation S...

K E Y W O R D S dentistry, enamel, fissure caries, confocal laser scanning microscopy, tomography, microradiography, acid fuchsin A C K N O W L E D G E M E N T S Thanks to Mr Y. Ito and Dr K. Masuno for their collaboration in the elaboration of histological slides and to Mr J. Fujita for his collaboration with English translation. A U T H O R D E TA I L S Dr T. Nishikawa, Department of Oral Pat...

Abstract To improve the resource utilization of solid waste, excavation abandoned soil and red mud, a by-product alumina industry, were introduced into preparation controlled low strength material (CLSM). By carrying out flowability test, bleeding test compressive relationship between properties CLSM mixture amount mud was analysed. The experiment results indicate that rate decrease with increa...

BACKGROUND Niemann Pick disease type C1 is a neurodegenerative disease caused by mutations in the NPC1 gene, which result in accumulation of unesterified cholesterol and glycosphingolipids in the endosomal-lysosomal system as well as limiting membranes. We have previously shown the corneal involvement in NPC1 pathology in form of intracellular inclusions in epithelial cells and keratocytes. The...

Abstract Confocal laser-scanning microscopy (CLSM) is one of the most popular optical architectures for fluorescence imaging. In CLSM, a focused laser beam excites emission from specific specimen position. Some actuators scan probed region across sample and photodetector collects single intensity value each point, building two-dimensional image pixel-by-pixel. Recently, new fast single-photon a...

PURPOSE To develop a novel in vitro method to detect the depth of penetration of the tear film protein albumin into contact lens materials using confocal laser scanning microscopy (CLSM). METHODS A poly-HEMA-based hydrogel (etafilcon A) and a silicone hydrogel material (lotrafilcon B) were examined. In vitro, bovine serum albumin (BSA) was labeled with 5-(4,6-dichloro-s-triazin-2-ylamino) flu...

Neuroendocrine (NE) differentiation is infrequent in testicular tumors and its histogenesis is not well understood. The present study is aimed at elucidating the pathway of neuroendocrine differentiation in germ cell tumors (GCTs) of the testis. In the analysis of 46 germ cell tumor components from 23 testicular tumors, we focused on GCTs with neuroendocrine differentiation, 7 teratoma, 1 embry...