Many forages experience significant proteolytic losses when preserved by ensiling. Such losses in alfalfa (Medicago sativa L.) are especially high, with degradation of 44 to 87% of the forage protein to nonprotein N (NPN). In contrast, red clover (Trifolium pratense L.) has up to 90% less proteolysis during ensiling. Here we demonstrate that the combination of polyphenol oxidase (PPO) and o-dip...

The improved Wilson quark action – the clover action – is constructed to have smaller discretization errors than the normal Wilson quark action. We test this in a quenched spectroscopy computation on 6 lattice ensembles with spacings from 0.15 to 0.43 fm. To ensure that the dominant scaling violations come from the fermions we use an O(a) improved 6-link SU(3) pure gauge action. We find evidenc...

We have identified two Rhizobium meliloti genes (nodD2 and nodD3) that are highly homologous and closely linked to the regulatory gene nodD (nodD1). R. meliloti strains containing mutations in the three nodD genes in all possible combinations were constructed and their nodulation phenotypes were assayed on Medicago sativa (alfalfa) and Melilotus alba (sweet clover). A triple nodD1-nodD2-nodD3 m...

The data provide an overview of proteomic changes in red clover (Trifolium pratense L.) in response to cold acclimation and recurrent selection for superior freezing tolerance. Proteins were extracted from crowns of two red clover cultivars grown under non-acclimated or cold-acclimated conditions, and plants obtained from the initial genetic background (TF0) and from populations obtained after ...

Cell culture and genetic engineering techmques can be used to develop improved pasture plants. To utltise these methods we have developed procedures for regenerating plants from tissue cultures of perennial ryegrass and white clover. In both, the plant genotype influences regeneration capacity. There was significant genetic variation among regenerated perennial ryegrass plants in a wde range of...

The transcription of four members of the Kunitz proteinase inhibitor (KPI) gene family of white clover (Trifolium repens L.), designated as Tr-KPI1, Tr-KPI2, Tr-KPI4 and Tr-KPI5, was investigated at both local infection (roots) and systemic (leaf tissue) sites in white clover in response to infection with the clover root knot nematode (CRKN) Meloidogyne trifoliophila and the clover cyst nematod...

The isolation of small amounts of genistein (5:7:4'trihydroxy-isoflavone) from subterranean clover (Trifolium subterraneum var. Dwalganup) and the demonstration of its weakly oestrogenic properties (Bradbury & White, 1951, and Biggers & Curnow, 1954), together with the difficulties in the synthesis of this compound, made it important that a more efficient method be found for the isolation of th...

Either NO(3) (-) (16 millimolar) or NH(4) (+) (1 millimolar) completely inhibited infection and nodulation of white clover seedlings (Trifoliin repens) inoculated with Rhizobium trifolii. The binding of R. trifolii to root hairs and the immunologically detectable levels of the plant lectin, trifoliin, on the root hair surface had parallel declining slopes as the concentration of either NO(3) (-...

Seeds of alfalfa (Medicago sativa) and white clover (Trifolium repens) were primed in 3 priming solutions [2% NaCl, 2% KNO3 and 300 g/L polyethylene glycol (PEG)] for 2 days at 20°C and their subsequent germination compared with that of untreated seed. Four replications of 50 seeds for germination and 25 seeds for emergence were arranged in a 2-factor factorial completely randomised design. All...

Soybean Dwarf Virus (SbDV) is an important plant pathogen, causing economic losses in soybean. In North America, indigenous strains of SbDV mainly infect clover, with occasional outbreaks in soybean. To evaluate the risk of a US clover strain of SbDV adapting to other plant hosts, the clover isolate SbDV-MD6 was serially transmitted to pea and soybean by aphid vectors. Sequence analysis of SbDV...