We report the designing of three expression vectors that can be used for rapid cloning of any blunt-end DNA segment. Only a single set of oligonucleotides are required to perform the amplification of the target DNA and its cloning in all three vectors simultaneously. The DNA thus cloned can express a protein either with or without a hexa-histidine tag depending upon the vector used. The express...

It is well known that it impossible to clone an arbitrary quantum state. However, this inability does not lead directly no-cloning of coherence. Here, we show the coherence state which a stronger statement than 'no-cloning state'. In particular, with ancillary system as machine state, states whose greater on transformations are defined. Also, characterize class for cloning will be possible give...

Now a days Copy and Paste of code fragments has been regularly practiced in development of software. Because of limitations of time and lack of knowledge programmers use this code strategy. This strategy is known as code cloning. Clones may cause many problems. Probability of errors and the maintenance cost is increased. Modification would be difficult because of clones. So it needs to detect c...

We describe a novel expression cloning strategy in the fission yeast for the isolation of mammalian transcription factors using a mammalian promoter as target. This strategy is possible because of the conservation between mammalian cells and Schizosaccharomyces pombe of the mechanism that leads to the selection of the transcription start site. It also opens new perspectives to investigate the t...

II A beautiful idea 2 A The intuition . . . . . . . . . . . . . . . 2 B Classical cryptography . . . . . . . . . 3 1 Asymmetrical (public-key) cryptosystems . . . . . . . . . . . . . . . 3 2 Symmetrical (secret-key) cryptosystems . . . . . . . . . . . . . . . . . 4 3 The one-time-pad as “classical teleportation” . . . . . . . . . . . . . . 5 C The example of the BB84 protocol . . . 5 1 Principl...

II A beautiful idea 2 A The intuition . . . . . . . . . . . . . . . 2 B Classical cryptography . . . . . . . . . 3 1 Asymmetrical (public-key) cryptosystems . . . . . . . . . . . . . . . 3 2 Symmetrical (secret-key) cryptosystems . . . . . . . . . . . . . . . . . 4 3 The one-time-pad as “classical teleportation” . . . . . . . . . . . . . . 5 C The example of the BB84 protocol . . . 5 1 Principl...

Monoclonal antibodies (mAbs) have well established their valuable roles as therapeutic proteins. Second generation of mAbs as therapeutical products for immunotherapy of HIV infection has made significant progress in recent studies. A recent small phase 1 clinical trial has demonstrated that monoclonal antibodies could be safe and effective in reducing HIV-1 viraemia in human. This study also s...

The importance of the IgE response in protection against infection by schistosomes has been demonstrated in both animal models and in man [1,2]. Among the targets of this response [3], the potential role of a protein of 22 kDa has been emphasized [4] and molecules of 20-22 kDa are widely recognized by IgE in human infection sera (manuscript in preparation). We therefore developed a cloning stra...

An image registration approach based on Evolution Strategies is proposed. In image registration, an invariant reference needs to be established within each source image, which is unavailable in many cases. To solve this problem, feature configuration (which is defined as the cluster of feature vectors on an image representing homogeneous feature distribution,) is employed to describe the object...